{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T06:42:10Z","timestamp":1792478530095,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pigment Cell Melanoma Res"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Melanoma is a highly aggressive skin tumour that poses considerable challenges in treatment and prognostic assessment. Epithelial\u2013mesenchymal transition (EMT) is a critical process in melanoma progression and contributes to tumour invasion and metastasis. This study aimed to identify EMT\u2010related genes associated with melanoma prognosis and to develop a risk model for patient stratification. Based on previous studies, an EMT\u2010related gene set comprising 200 genes was compiled. Gene expression data from normal skin and skin cutaneous melanoma tissues were obtained from The Cancer Genome Atlas (TCGA) and Genotype\u2010Tissue Expression (GTEx) databases. Differential expression analysis was performed to identify differentially expressed genes (DEGs), using an adjusted\n                    <jats:italic>p<\/jats:italic>\n                    value &lt;\u20090.05 as the significance threshold. Univariable Cox regression analysis was subsequently conducted to identify survival\u2010related genes among the DEGs (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05), resulting in the selection of six genes: LAMC2, IL32, MGP, CAP2, ECM1 and BGN. A risk score model was then constructed based on these genes. The model showed prognostic stratification ability in both the TCGA cohort and the external validation cohort GSE65904. Patients were classified into high\u2010 and low\u2010risk groups, and overall survival was significantly shorter in the high\u2010risk group than in the low\u2010risk group (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). Analysis of the immune microenvironment showed higher levels of immune cell infiltration in the low\u2010risk group. BGN was significantly overexpressed in melanoma tissues and cell lines. In\u00a0vitro experiments demonstrated that BGN silencing inhibited the proliferation, migration and invasion of A375 cells and reversed EMT\u2010related changes, whereas BGN overexpression enhanced the malignant phenotype of A2058 cells. In\u00a0vivo experiments further confirmed that BGN knockdown significantly suppressed xenograft tumour growth and altered the expression of EMT\u2010related markers. These findings establish an EMT\u2010related prognostic model for melanoma risk stratification and provide experimental evidence supporting the potential role of BGN in melanoma progression. This study integrates computational analysis with functional validation; however, further validation in independent prospective cohorts is required before clinical application.\n                  <\/jats:p>","DOI":"10.1111\/pcmr.70132","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T06:22:28Z","timestamp":1792477348000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Construction of an\n                    <scp>EMT<\/scp>\n                    \u2010Related Prognostic Model for Melanoma and Functional Validation of the\n                    <scp>EMT<\/scp>\n                    \u2010Associated Gene\n                    <scp>BGN<\/scp>"],"prefix":"10.1111","volume":"39","author":[{"given":"Na","family":"Zhao","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Zhengzhou University  Zhengzhou P. R. China"},{"name":"Henan Key Laboratory of Tumor Pathology Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5328-0646","authenticated-orcid":false,"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Dongguan Hospital of Guangzhou University of Chinese Medicine  Dongguan P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhui","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Pathology Zhengzhou Central Hospital Affiliated to Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chenbo","family":"Yang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Zhengzhou University  Zhengzhou P. R. China"},{"name":"Henan Key Laboratory of Tumor Pathology Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miaomiao","family":"Sun","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Zhengzhou University  Zhengzhou P. R. China"},{"name":"Henan Key Laboratory of Tumor Pathology Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6233-9665","authenticated-orcid":false,"given":"Guangwen","family":"Yin","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Zhengzhou University  Zhengzhou P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010\u2010021\u201004216\u2010z"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078\u20100432.CCR\u201022\u20101922"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2021108647"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2024.06.001"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43018\u2010023\u201000594\u2010z"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3002487"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2023.116472"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.63093"},{"key":"e_1_2_9_10_1","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1016\/S0955-0674(89)80072-9","article-title":"The Extended Family of Proteoglycans: Social Residents of the Pericellular Zone","volume":"1","author":"Gallagher J. 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