{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T16:12:42Z","timestamp":1788365562850,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["R01MH107183"],"award-info":[{"award-number":["R01MH107183"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["R01MH118884"],"award-info":[{"award-number":["R01MH118884"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["R01MH124248"],"award-info":[{"award-number":["R01MH124248"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["R01MH128994"],"award-info":[{"award-number":["R01MH128994"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["R01MH130539"],"award-info":[{"award-number":["R01MH130539"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000025","name":"National Institute of Mental Health","doi-asserted-by":"publisher","award":["R56MH138596"],"award-info":[{"award-number":["R56MH138596"]}],"id":[{"id":"10.13039\/100000025","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Psychiatry Clin Neurosci"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Major depressive disorder (MDD) is a leading cause of disability and carries a high risk of suicide. MicroRNAs (miRNAs) are epigenetic regulators implicated in MDD and can be regulated by DNA methylation, potentially reshaping downstream gene networks. We investigated methylation\u2010linked miRNA dysregulation and explored whether these changes are specifically associated with suicide among MDD patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Genome\u2010wide DNA methylation profiling of the dorsolateral prefrontal cortex from 15 MDD patients who died by suicide (MDD+S), 17 MDD patients who died from causes other than suicide (MDD\u2212S), and 16 controls (C) using the Illumina 850K MethylationEPIC array was integrated with small RNA sequencing\u2010based miRNA quantification to identify miRNA\u2010associated differentially methylated probes (DMPs), link methylation to miRNA expression, and infer downstream targets and pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Differential methylation analysis (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009\u2264\u20090.05) revealed 139 miRNA\u2010linked DMPs in C\n                      <jats:italic>vs.<\/jats:italic>\n                      MDD+\/\u2212S, 135 in C\n                      <jats:italic>vs.<\/jats:italic>\n                      MDD\u2212S, 179 in C\n                      <jats:italic>vs.<\/jats:italic>\n                      MDD+S, and the highest in MDD+S\n                      <jats:italic>vs.<\/jats:italic>\n                      MDD\u2212S (235). CpG\u2010miRNA pairing (within 1500kb promoter) followed by Spearman correlation identified inverse associations between CpG \u03b2 values and miRNA expression, with the most consistent signals in suicide\u2010status\u2010stratified subsets, including cg06341821\u2010hsa\u2010miR\u2010574\u20103p and cg25451306\u2010hsa\u2010miR\u20102110 in MDD+S\u2010related contrasts, and cg06179179\u2010hsa\u2010miR\u2010595 in MDD\u2212S\u2010related contrasts. High\u2010confidence target prediction and ClueGO enrichment indicated distinct biology: miR\u2010595 target genes in MDD\u2212S were enriched for interferon\/innate immune signaling, whereas miR\u20102110 target genes in MDD+S were enriched for ligand\u2010gated ion channel activity and synaptic\/receptor signaling.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This integrated approach identifies methylation\u2010regulated miRNA pathways that may play key roles in the molecular pathogenesis of MDD and suicide.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/pcn.70090","type":"journal-article","created":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T03:56:16Z","timestamp":1780026976000},"page":"757-764","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrative methylation and\n                    <scp>miRNA<\/scp>\n                    dysregulation in\n                    <scp>dlPFC<\/scp>\n                    reveal distinct molecular signatures of suicide and non\u2010suicide subtypes in major depressive disorder"],"prefix":"10.1111","volume":"80","author":[{"given":"Aleena","family":"Francis","sequence":"first","affiliation":[{"name":"Department of Psychiatry and Behavioral Neurobiology, Heersink School of Medicine University of Alabama at Birmingham  Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5359-4717","authenticated-orcid":false,"given":"Yogesh","family":"Dwivedi","sequence":"additional","affiliation":[{"name":"Department of Psychiatry and Behavioral Neurobiology, Heersink School of Medicine University of Alabama at Birmingham  Birmingham Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0033291721000441"},{"key":"e_1_2_11_3_1","volume-title":"Major Depressive Disorder","author":"Bains N","year":"2024"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-025-01549-x"},{"key":"e_1_2_11_5_1","first-page":"8","article-title":"Epigenetic regulation in major depression and other stress\u2010related disorders: Molecular mechanisms, clinical relevance and therapeutic potential","volume":"8","author":"Yuan ML","year":"2023","journal-title":"Signal Transduct. 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