{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,29]],"date-time":"2024-02-29T19:06:05Z","timestamp":1709233565461},"reference-count":9,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T00:00:00Z","timestamp":1373328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Dermatology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Even though prompt diagnosis and treatment of purpura fulminans (<jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content>) is essential to reduce mortality, early administration of antibiotics may preclude identification of the causative agent by standard bacterial cultures and thus render definitive diagnosis impossible. Here we present a case of an infant with <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content> and negative bacterial cultures for whom polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) analysis of a cutaneous biopsy specimen obtained 4\u00a0days after initiation of antibiotics identified the genomic sequence of <jats:italic><jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>eisseria meningitidis<\/jats:italic> genogroup <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>. When bacterial cultures fail to provide useful information, <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> of skin biopsy specimens can be a valuable diagnostic tool in <jats:styled-content style=\"fixed-case\">PF<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/pde.12181","type":"journal-article","created":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T08:16:48Z","timestamp":1373357808000},"source":"Crossref","is-referenced-by-count":3,"title":["Diagnostic Value of Polymerase Chain Reaction Analysis of Skin Biopsies in Purpura Fulminans"],"prefix":"10.1111","volume":"30","author":[{"given":"Caroline","family":"Beau","sequence":"first","affiliation":[{"name":"Pediatric Dermatology Unit H\u00f4pital Pellegrin\u2010Enfants Bordeaux France"},{"name":"Department of Pediatrics H\u00f4pital Pellegrin\u2010Enfants Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natalia","family":"Vlassova","sequence":"additional","affiliation":[{"name":"Pediatric Dermatology Unit H\u00f4pital Pellegrin\u2010Enfants Bordeaux France"},{"name":"Mercy Medical Center Baltimore Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Sarlangue","sequence":"additional","affiliation":[{"name":"Department of Pediatrics H\u00f4pital Pellegrin\u2010Enfants Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Brissaud","sequence":"additional","affiliation":[{"name":"Department of Pediatrics H\u00f4pital Pellegrin\u2010Enfants Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"L\u00e9aut\u00e9\u2010Labr\u00e8ze","sequence":"additional","affiliation":[{"name":"Pediatric Dermatology Unit H\u00f4pital Pellegrin\u2010Enfants Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Franck","family":"Boralevi","sequence":"additional","affiliation":[{"name":"Pediatric Dermatology Unit H\u00f4pital Pellegrin\u2010Enfants Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,7,9]]},"reference":[{"key":"e_1_2_4_2_1","first-page":"300","volume-title":"Hurwitz clinical pediatric dermatology","author":"Paller A","year":"2011"},{"key":"e_1_2_4_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.2010.199919"},{"key":"e_1_2_4_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2458-9-95"},{"key":"e_1_2_4_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-007-0667-2"},{"key":"e_1_2_4_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1553-1558.2001"},{"key":"e_1_2_4_7_1","volume-title":"PCR for detection and characterization of bacterial meningitis pathogens: Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae. Laboratory methods for the diagnosis of meningitis","author":"Centers for Disease Control and Prevention","year":"2011"},{"key":"e_1_2_4_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.144.6.770"},{"key":"e_1_2_4_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)67932-4"},{"key":"e_1_2_4_10_1","first-page":"175","article-title":"17th Consensus conference. Consensus conference on bacterial meningitis. Short text","volume":"39","author":"Soci\u00e9t\u00e9 de pathologie infectieuse de langue fran\u00e7aise","year":"2009","journal-title":"Med Mal Infect"}],"container-title":["Pediatric Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpde.12181","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pde.12181","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T00:20:32Z","timestamp":1696033232000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pde.12181"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,9]]},"references-count":9,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1111\/pde.12181"],"URL":"https:\/\/doi.org\/10.1111\/pde.12181","archive":["Portico"],"relation":{},"ISSN":["0736-8046","1525-1470"],"issn-type":[{"value":"0736-8046","type":"print"},{"value":"1525-1470","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,9]]}}}