{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:40:15Z","timestamp":1786120815201,"version":"build-2736575974"},"reference-count":7,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T00:00:00Z","timestamp":1785974400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Dermatology"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Vaccination\u2010induced granuloma is a common clinical diagnosis, but few reports have described the diagnostic utility of comprehensive pathological and microbiological examinations. This report details a case of DTaP\u2010IPV vaccination\u2010induced granuloma where the use of multi\u2010virus\/microbial real\u2010time polymerase chain reaction (PCR) enabled a definitive molecular diagnosis. This advanced testing method confirmed the presence in the excised tissue of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Bordetella pertussis<\/jats:italic>\n                    <\/jats:styled-content>\n                    and poliovirus genes from the vaccine components, a novel finding for this type of lesion. In cases requiring a definitive diagnosis, especially those where malignancy is a differential, surgical excision followed by multiplex PCR testing is a reliable option.\n                  <\/jats:p>","DOI":"10.1111\/pde.70333","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T01:32:52Z","timestamp":1786066372000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>DTaP<\/scp>\n                    \u2010\n                    <scp>IPV<\/scp>\n                    Vaccination\u2010Induced Granuloma Diagnosed With Multi\u2010Virus\/Microbial Real\u2010Time\n                    <scp>PCR<\/scp>"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-216X","authenticated-orcid":false,"given":"Hiroshi","family":"Hayashi","sequence":"first","affiliation":[{"name":"Department of Hematology\/Oncology Tokyo Metropolitan Children's Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuto","family":"Otsubo","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Pediatrics Tokyo Metropolitan Children's Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8687-7625","authenticated-orcid":false,"given":"Kentaro","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Tokyo Metropolitan Children's Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5875-1024","authenticated-orcid":false,"given":"Yuho","family":"Horikoshi","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Pediatrics Tokyo Metropolitan Children's Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5332-4434","authenticated-orcid":false,"given":"Harutaka","family":"Katano","sequence":"additional","affiliation":[{"name":"Department of Pathology National Institute of Infectious Diseases  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Yuza","sequence":"additional","affiliation":[{"name":"Department of Hematology\/Oncology Tokyo Metropolitan Children's Medical Center  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,6]]},"reference":[{"key":"e_1_2_1_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.161"},{"key":"e_1_2_1_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21962"},{"issue":"11","key":"e_1_2_1_8_4_1","first-page":"15479","article-title":"Development of a New Real\u2010Time PCR System for Simultaneous Detection of Bacteria and Fungi in Pathological Samples","volume":"8","author":"Fukumoto H.","year":"2015","journal-title":"International Journal of Clinical and Experimental Pathology"},{"key":"e_1_2_1_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/pde.13732"},{"key":"e_1_2_1_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/cod.14180"},{"key":"e_1_2_1_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199305000-00012"},{"key":"e_1_2_1_8_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamadermatol.2022.0828"}],"container-title":["Pediatric Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pde.70333","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pde.70333","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pde.70333","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T01:32:53Z","timestamp":1786066373000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pde.70333"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,6]]},"references-count":7,"alternative-id":["10.1111\/pde.70333"],"URL":"https:\/\/doi.org\/10.1111\/pde.70333","archive":["Portico"],"relation":{},"ISSN":["0736-8046","1525-1470"],"issn-type":[{"value":"0736-8046","type":"print"},{"value":"1525-1470","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,6]]},"assertion":[{"value":"2025-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"pde.70333"}}