{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T16:36:56Z","timestamp":1751387816011},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,12,11]],"date-time":"2014-12-11T00:00:00Z","timestamp":1418256000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatrics International"],"published-print":{"date-parts":[[2015,4]]},"abstract":"Abstract<\/jats:title>Background<\/jats:title>The appropriate choice of antibiotics against M<\/jats:styled-content>ycoplasma pneumoniae<\/jats:italic> infection has become difficult, as the prevalence of macrolide\u2010resistant M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic> has increased.<\/jats:p><\/jats:sec>Methods<\/jats:title>Throat swab specimens were collected from children with clinically suspected M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic> infection while visiting an outpatient clinic. Cultures for M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic> were done, and all isolates were sequenced for the presence of a mutation in 23S rRNA<\/jats:styled-content><\/jats:italic>.<\/jats:p><\/jats:sec>Results<\/jats:title>Of the 80 specimens collected between F<\/jats:styled-content>ebruary 2012 and M<\/jats:styled-content>arch 2013, 27 (34%) were positive for M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic> on culture. Macrolide\u2010resistant mutation was detected in 24 isolates (89%): 23 isolates had an A<\/jats:styled-content>2063G<\/jats:styled-content> transition, and one had a C<\/jats:styled-content>2617G<\/jats:styled-content> mutation. Both the median age and the prevalence of pneumonia were significantly higher in M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic>\u2010positive than in M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic>\u2010negative children (median, 7 years vs 4 years; 88.9% vs 60.4%, respectively). The percentage of serum samples with particle agglutination titer \u22651:160 was 69.6% in M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic>\u2010positive cases and 17.6% in M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic>\u2010negative cases when the serum was collected \u22654\u2009days after the onset of fever. Defervescence within 72\u2009h after the initiation of macrolides never occurred in M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic>\u2010positive children and also did not occur in 54% of M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic>\u2010negative children. Switching to either minocycline or tosufloxacin resulted in fever resolution within 48\u2009h in M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic>\u2010positive children.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The described clinical and laboratory characteristics of M<\/jats:styled-content>.\u2009pneumoniae<\/jats:italic> infection may be useful in guiding appropriate treatment in an outpatient clinic.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ped.12513","type":"journal-article","created":{"date-parts":[[2014,9,29]],"date-time":"2014-09-29T12:33:14Z","timestamp":1411993994000},"page":"247-252","source":"Crossref","is-referenced-by-count":13,"title":["Characteristics of M<\/scp>ycoplasma pneumoniae<\/i> infection identified on culture in a pediatric clinic"],"prefix":"10.1111","volume":"57","author":[{"given":"Yuriko","family":"Katsushima","sequence":"first","affiliation":[{"name":"Katsushima Pediatric Clinic Yamagata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fumio","family":"Katsushima","sequence":"additional","affiliation":[{"name":"Katsushima Pediatric Clinic Yamagata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Microbiology Yamagata Prefectural Institute of Public Health Yamagata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junji","family":"Seto","sequence":"additional","affiliation":[{"name":"Department of Microbiology Yamagata Prefectural Institute of Public Health Yamagata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsumi","family":"Mizuta","sequence":"additional","affiliation":[{"name":"Department of Microbiology Yamagata Prefectural Institute of Public Health Yamagata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidekazu","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Virus Research Center Clinical Research Division Sendai Medical Center Miyagi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoko","family":"Matsuzaki","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases Yamagata University Faculty of Medicine Yamagata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,12,11]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2001.tb01293.x"},{"key":"e_1_2_6_3_1","first-page":"337","article-title":"Large epidemic of mycoplasmal pneumonia caused by Mycoplasma pneumoniae strains highly resistant to macrolides among children in 2011","volume":"32","author":"Ubukata K","year":"2011","journal-title":"Infect. 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