{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T12:52:30Z","timestamp":1760100750683},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,7,14]],"date-time":"2015-07-14T00:00:00Z","timestamp":1436832000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatrics International"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Hepatoblastoma is a rare childhood malignant tumor that originates from immature hepatic cells. Aminopeptidase\u2010<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>(<jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content>), an ectopeptidase that promotes tumor invasion and metastasis, is expressed in fetal stage hepatic progenitor cells, although its role in hepatoblastoma remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression pattern of <jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content> was investigated on immunohistochemistry in 30 tissue samples from 27 hepatoblastoma patients (16 with predominantly embryonal [<jats:styled-content style=\"fixed-case\">pE<\/jats:styled-content>] histology and 14 with predominantly fetal [<jats:styled-content style=\"fixed-case\">pF<\/jats:styled-content>] histology). Immunoreactive score (<jats:styled-content style=\"fixed-case\">IRS<\/jats:styled-content>) was used to quantify staining data, and the relationship between <jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content> expression, clinicopathological factors, and clinical outcome was investigated. The biological function of <jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content> was also examined in the hepatoblastoma cell lines <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>uh6 and <jats:styled-content style=\"fixed-case\">HepG2<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All specimens stained positive for <jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content>, with higher <jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content> expression in <jats:styled-content style=\"fixed-case\">pE<\/jats:styled-content> than in <jats:styled-content style=\"fixed-case\">pF<\/jats:styled-content> hepatoblastoma samples (median <jats:styled-content style=\"fixed-case\">IRS<\/jats:styled-content>, 4; range, 2\u20139 vs 2; range, 1\u20134). Strong <jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content> expression was correlated with vascular invasion. Five\u2009year event\u2010free survival and overall survival were better in patients with <jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content><jats:sup>low<\/jats:sup> than in those with <jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content><jats:sup>high<\/jats:sup> tumors (100% vs 51.0%, <jats:italic>P<\/jats:italic> = 0.026; and 100% vs 74.0%, <jats:italic>P<\/jats:italic> = 0.114, respectively). A <jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content>\u2010neutralizing antibody and the potent <jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content> inhibitor, Ubenimex, suppressed invasive activity in <jats:styled-content style=\"fixed-case\">HepG2<\/jats:styled-content> cells <jats:italic>in vitro<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CD13<\/jats:styled-content> expression is associated with hepatoblastoma invasiveness and could be a novel prognostic marker for hepatoblastoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ped.12597","type":"journal-article","created":{"date-parts":[[2015,2,13]],"date-time":"2015-02-13T10:48:50Z","timestamp":1423824530000},"page":"558-566","source":"Crossref","is-referenced-by-count":9,"title":["Prognostic significance of aminopeptidase\u2010<scp>N<\/scp> (<scp>CD13<\/scp>) in 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