{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T05:42:59Z","timestamp":1772948579497,"version":"3.50.1"},"reference-count":45,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2016,8,8]],"date-time":"2016-08-08T00:00:00Z","timestamp":1470614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science, and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatrics International"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We investigated the effects of glucocorticoids, erythropoietin (EPO) and spironolactone (SPL) n human fetal lung fibroblasts and human alveolar epithelial cells exposed to tracheal aspirate fluid (TAF) from extremely premature infants with chronic lung disease (CLD), characterized by fibrosis and changes in the alveolar epithelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fibroblasts and epithelial cells (FHs 738Lu and A549, respectively) were treated with different concentrations of hydrocortisone (HDC), dexamethasone (DEX), betamethasone (BET), SPL, and EPO in the absence or presence of TAF from infants with CLD (gestational age, 25.3 \u00b1 0.8 weeks; birthweight, 658 \u00b1 77 g; postnatal age, 0\u201328 days) and assayed for proliferation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Exposure to TAF resulted in a concentration\u2010dependent proliferation of fibroblasts and epithelial cells. Proliferation of TAF\u2010exposed fibroblasts was suppressed most significantly by 100 \u03bcmol\/L DEX (21%, <jats:italic>P<\/jats:italic> = 0.046) and 300 mIU\/mL EPO (18%, <jats:italic>P<\/jats:italic> = 0.02) and promoted most significantly by 0.4 \u03bcmol\/L HDC (10%, <jats:italic>P<\/jats:italic> = 0.04). Epithelial proliferation was promoted by 4 \u03bcmol\/L HDC (15%, <jats:italic>P<\/jats:italic> = 0.04), 10 \u03bcmol\/L DEX (53%, <jats:italic>P<\/jats:italic> &lt; 0.01), 0.2 \u03bcmol\/L BET (56%, <jats:italic>P<\/jats:italic> &lt; 0.01), and 300 mIU\/mL EPO (35%, <jats:italic>P<\/jats:italic> &lt; 0.01) in the presence of TAF. Treatment with glucocorticoids alone did not significantly affect fibroblast proliferation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Glucocorticoids and EPO reduced fibroproliferation while promoting epithelial cell growth <jats:italic>in vitro<\/jats:italic> within certain dose ranges. Appropriate doses of glucocorticoids and EPO may be useful in the prevention and resolution of CLD in extremely premature infants.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ped.13009","type":"journal-article","created":{"date-parts":[[2016,4,14]],"date-time":"2016-04-14T05:34:57Z","timestamp":1460612097000},"page":"1163-1170","source":"Crossref","is-referenced-by-count":3,"title":["Glucocorticoids and erythropoietin in chronic lung disease of prematurity: Proliferative potential in lung fibroblast and epithelial cells exposed to tracheal aspirates"],"prefix":"10.1111","volume":"58","author":[{"given":"Satoshi","family":"Ohnishi","sequence":"first","affiliation":[{"name":"Department of Pediatrics Osaka City University Graduate School of Medicine  Osaka Japan"},{"name":"Department of Neonatology Osaka City General Hospital  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Ichiba","sequence":"additional","affiliation":[{"name":"Department of Neonatology Osaka City General Hospital  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mika","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Hamazaki","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisako","family":"Matsumura","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Osaka City University Graduate School of Medicine  Osaka Japan"},{"name":"Department of Neonatology Osaka City General Hospital  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruo","family":"Shintaku","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Osaka City University Graduate School of Medicine  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,8,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60074-4"},{"key":"e_1_2_8_3_1","volume-title":"Care of High Risk Neonate","author":"Klaus MH","year":"2001"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-200X.1996.tb03738.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(96)70355-4"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.110.4.768"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000047656.17766.39"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.159.5.9806073"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"3897","DOI":"10.1016\/S0021-9258(18)61441-3","article-title":"The effect of transforming growth factor\u2010\u03b21on cell proliferation and collagen formation by lung fibroblasts","volume":"15","author":"Fine A","year":"1987","journal-title":"J. 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