{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T11:46:54Z","timestamp":1784116014832,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2020,8,3]],"date-time":"2020-08-03T00:00:00Z","timestamp":1596412800000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatrics International"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>There have been few reports on the genetic structure of the current population of methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic> (MRSA) from neonatal intensive care units (NICUs) in Japan. In the present study we conducted a molecular epidemiological analysis based on whole genome sequencing against MRSA strains in a Japanese NICU.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed genotyping by whole genome sequencing, polymerase chain reaction\u2010based typing of <jats:italic>Staphylococcal<\/jats:italic> cassette chromosome <jats:italic>mec<\/jats:italic> (SCC<jats:italic>mec<\/jats:italic>) and polymerase chain reaction\u2010based open\u2010reading frame typing against 57 MRSA strains from fecal or nasal specimens from NICU patients in Juntendo University Shizuoka Hospital in 2013\u20132014.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Forty\u2010nine MRSA strains (86.0%) exhibited a clonal complex (CC) 1, and were divided into three sequence types (STs): ST2725 (<jats:italic>n<\/jats:italic> = 25), ST2764 (<jats:italic>n <\/jats:italic>= 21), and ST1 (<jats:italic>n<\/jats:italic> = 3). All CC1 MRSA strains had SCC<jats:italic>mec<\/jats:italic> IVa, and were resistant to new quinolones, which are limited in pediatric use, suggesting that these strains were derived from adult MRSA clones. Single nucleotide polymorphism differences of both \u226410 and &gt;100 nucleotides were observed by pairwise, single nucleotide polymorphism analysis among ST2725 and ST2764 MRSA strains, respectively. Seven ST8 MRSA strains (12.2%) were isolated, and no strain exhibiting the Japanese hospital\u2010associated MRSA genotype (ST5\/SCC<jats:italic>mec<\/jats:italic> II) was isolated in this study.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our molecular epidemiological analysis suggested that ST2725 and ST2764 MRSA strains had genetic diversity that could not be explained only by a recent transmission event in the NICU. These MRSA clones might be disseminated in other Japanese hospital facilities as new endemic clones. Our results are expected to contribute to the improvement of infection control measures of MRSA in NICUs.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ped.14241","type":"journal-article","created":{"date-parts":[[2020,3,26]],"date-time":"2020-03-26T04:30:25Z","timestamp":1585197025000},"page":"911-919","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Epidemiology of methicillin\u2010resistant <i>Staphylococcus aureus<\/i> in a Japanese neonatal intensive care unit"],"prefix":"10.1111","volume":"62","author":[{"given":"Atsushi","family":"Tsujiwaki","sequence":"first","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3618-883X","authenticated-orcid":false,"given":"Ken","family":"Hisata","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yudai","family":"Tohyama","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuaki","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Uehara","sequence":"additional","affiliation":[{"name":"Department of Infection Control Science Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Department of Infection Control Science Juntendo University Graduate School of Medicine Tokyo Japan"},{"name":"Animal Research Center Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keiichi","family":"Hiramatsu","sequence":"additional","affiliation":[{"name":"Department of Infection Control Science Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0364-0022","authenticated-orcid":false,"given":"Toshiaki","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine Juntendo University Graduate School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.14.4.385"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.122108599"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/520437"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3947\/ic.2013.45.2.117"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.6.1449"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.158.2.513-516.1984"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.28.3.397"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1008-1015.2000"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.11.3556-3563.1999"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2006.02932.x"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.JJID.2009.386"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00020-18"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06094-11"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3364-3372.2005"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-018-3646-z"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2017.06.021"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2013-3413"},{"key":"e_1_2_10_19_1","volume-title":"Performance standards for antimicrobial susceptibility testing; Twenty\u2010Sixth Informational Supplement. 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