{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:43:27Z","timestamp":1753879407080,"version":"3.41.2"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,4,9]],"date-time":"2022-04-09T00:00:00Z","timestamp":1649462400000},"content-version":"vor","delay-in-days":98,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatrics International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The effects of therapeutic hypothermia (TH) on renal function are not widely reported, especially in longer term animal models. The hypothesis of this study was that TH of the kidneys of hypoxic\u2013ischemic newborn piglets would reduce pathological renal fibrosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010five newborn piglets obtained within 24\u2009h of birth were classified into a control group (<jats:italic>n<\/jats:italic>\u2009=\u20095), an hypoxic insult with normothermia (HI\u2010NT) group (<jats:italic>n<\/jats:italic>\u2009=\u200912), and an hypoxic insult with TH (HI\u2010TH) group (33.5 \u00b0C\u2009\u00b1\u20090.5 \u00b0C for 24\u2009h; <jats:italic>n<\/jats:italic>\u2009=\u20098). Five days after the insult, all piglets were sacrificed under deep anesthesia by isoflurane inhalation. The kidneys were perfused with phosphate\u2010buffered paraformaldehyde and immersed in formalin buffer. Territory fibrosis was studied and scored in the renal medulla using Azan staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fibrosis area scores (means \u00b1 standard deviations) based on Azan staining were 1.00\u2009\u00b1\u20090.46 in the control group, 2.85\u2009\u00b1\u20090.93 in the HI\u2010NT group, and 3.58\u2009\u00b1\u20091.14 in the HI\u2010TH group. The fibrosis area of the HI\u2010NT and HI\u2010TH groups was larger than that of the control. The HI\u2010NT and HI\u2010TH groups were not statistically different.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Renal fibrosis is affected by perinatal asphyxia and cannot be prevented by TH, based on histopathological findings.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ped.14961","type":"journal-article","created":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T05:15:54Z","timestamp":1629436554000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Hypothermia cannot ameliorate renal fibrosis after asphyxia in the newborn piglet"],"prefix":"10.1111","volume":"64","author":[{"given":"Takayuki","family":"Wakabayashi","sequence":"first","affiliation":[{"name":"Department of Pediatrics Faculty of Medicine Kagawa University Miki\u2010cho 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