{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:47:53Z","timestamp":1760647673668,"version":"3.41.2"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,11,2]],"date-time":"2022-11-02T00:00:00Z","timestamp":1667347200000},"content-version":"vor","delay-in-days":305,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatrics International"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Iron deficiency during infancy is associated with poor neurological development, but iron overload causes severe complications. Appropriate iron supplementation is therefore vital. Reticulocyte hemoglobin content (RET\u2010He) provides a real\u2010time assessment of iron status and chracterezes hemoglobin synthesis in preterm infants. However, the existing literature lacks detailed reports assessing chronological changes in RET\u2010He. The aim of this study was to assess the chronological changes in RET\u2010He during oral iron dietary supplementation, and concomitant therapy with recombinant human erythropoietin (rHuEPO) in preterm very low birthweight infants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Very low birthweight infants, admitted to our neonatal intensive care unit were analyzed retrospectively. Hemoglobin (Hb), reticulocyte percentage (Ret), mean corpuscular volume, RET\u2010He, serum iron (Fe), and serum ferritin were recorded. Data at birth (T0), the initial day of rHuEPO therapy (T1), the initial day of oral iron supplementation (T2), 1\u20132\u2009weeks (T3), 3\u20134\u2009weeks (T4), 5\u20136\u2009weeks (T5), and 7\u20138\u2009weeks (T6) from the initial day of oral iron supplementation were extracted, and their changes over time were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Reticulocyte hemoglobin content was highest at birth and declined rapidly thereafter, especially after starting rHuEPO therapy. There was no upward trend in RET\u2010He after the initiation of oral iron supplementation, with a slower increase during 5\u20136\u2009weeks after the initiation of iron therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>During the treatment of anemia of prematurity, low RET\u2010He levels may be prolonged. Anemia of prematurity should therefore be assessed and treated on a case\u2010by\u2010case basis, while considering the iron metabolic capacity of preterm infants.<\/jats:p><\/jats:sec>","DOI":"10.1111\/ped.15330","type":"journal-article","created":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T15:09:54Z","timestamp":1660576194000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Reticulocyte hemoglobin content changes after treatment of anemia of prematurity"],"prefix":"10.1111","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0268-7216","authenticated-orcid":false,"given":"Mitsumaro","family":"Nii","sequence":"first","affiliation":[{"name":"Division of Neonatology Center for Maternity and Infant Care, Asahikawa Medical University Hospital  Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3244-0433","authenticated-orcid":false,"given":"Toshio","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Division of Neonatology Center for Maternity and Infant Care, Asahikawa Medical University Hospital  Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsutoshi","family":"Sugiyama","sequence":"additional","affiliation":[{"name":"Division of Neonatology Center for Maternity and Infant Care, Asahikawa Medical University Hospital  Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiko","family":"Aoyama","sequence":"additional","affiliation":[{"name":"Division of Neonatology Center for Maternity and Infant Care, Asahikawa Medical University Hospital  Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Nagaya","sequence":"additional","affiliation":[{"name":"Division of Neonatology Center for Maternity and Infant Care, Asahikawa Medical University Hospital  Hokkaido Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,11,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1997.03540360041028"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2010-2576"},{"volume-title":"Iron Deficiency Anaemia. 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