{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:58:09Z","timestamp":1790913489168,"version":"4.1.0"},"reference-count":8,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T00:00:00Z","timestamp":1749427200000},"content-version":"vor","delay-in-days":159,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatrics International"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Postnatal cytomegalovirus (CMV) infection, primarily transmitted through breast milk, poses significant risks for very preterm infants (VPIs), potentially leading to sepsis\u2010like syndromes, necrotizing enterocolitis, and pneumonia. Although pasteurization of breast milk has been shown to be effective in preventing postnatal CMV infection, no studies in Japan have examined its clinical efficacy in practice. This preliminary study investigated the incidence of postnatal CMV infection in VPIs born to CMV\u2010seropositive mothers who received pasteurized breast milk at our Japanese perinatal center.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>VPIs born before 32\u2009weeks' gestational age (GA) from September 2019 to August 2023, excluding those born to CMV\u2010seronegative or untested mothers, were retrospectively analyzed. Frozen\u2013thawed breast milk was sterilized by Holder pasteurization at 63\u00b0C for 30\u2009min. Urine CMV polymerase chain reaction (PCR) testing was routinely performed within the first 3\u2009weeks of life and again at 36\u2009weeks' corrected GA to assess CMV infection status.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 56 VPIs born to CMV\u2010seropositive mothers underwent urine CMV PCR at 36\u2009weeks' corrected GA to evaluate postnatal CMV infection. Among them, three cases (5.4%) were diagnosed with postnatal CMV infection; however, it remains unclear whether these cases were attributable to breast milk transmission or blood transfusions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This preliminary study is the first in Japan to demonstrate that the incidence of postnatal CMV infection in VPIs born to CMV\u2010seropositive mothers after receiving breast milk sterilized by Holder pasteurization was only 5.4%. These results support the effectiveness of our pasteurization protocol in preventing postnatal CMV infection.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ped.70076","type":"journal-article","created":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T10:10:16Z","timestamp":1749463816000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Breast milk pasteurization for preventing cytomegalovirus infection: A preliminary study in a Japanese perinatal center"],"prefix":"10.1111","volume":"67","author":[{"given":"Sakiko","family":"Uho","sequence":"first","affiliation":[{"name":"Department of Neonatology Hyogo Prefectural Kobe Children's Hospital Perinatal Center  Chuo\u2010ku, Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2871-4000","authenticated-orcid":false,"given":"Sota","family":"Iwatani","sequence":"additional","affiliation":[{"name":"Department of Neonatology Hyogo Prefectural Kobe Children's Hospital Perinatal Center  Chuo\u2010ku, Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shohei","family":"Ohyama","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Akashi Medical Center  Akashi Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Ikuta","sequence":"additional","affiliation":[{"name":"Department of Neonatology Hyogo Prefectural Kobe Children's Hospital Perinatal Center  Chuo\u2010ku, Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sachiko","family":"Matsui","sequence":"additional","affiliation":[{"name":"Department of Neonatology Hyogo Prefectural Kobe Children's Hospital Perinatal Center  Chuo\u2010ku, Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiji","family":"Yoshimoto","sequence":"additional","affiliation":[{"name":"Department of Neonatology Hyogo Prefectural Kobe Children's Hospital Perinatal Center  Chuo\u2010ku, Kobe Hyogo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamapediatrics.2014.1360"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.14920"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/ped.14458"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/bfm.2014.0108"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy945"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8020289"},{"key":"e_1_2_10_8_1","first-page":"1273","article-title":"Cytomegalovirus seropositivity in pregnant women in Japan during 1996\u20132009","volume":"46","author":"Azuma H","year":"2010","journal-title":"J Jpn Soc Perinat Neonat Med"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1203\/PDR.0b013e3181b4554a"}],"container-title":["Pediatrics International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ped.70076","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ped.70076","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ped.70076","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:49:14Z","timestamp":1790912954000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ped.70076"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":8,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/ped.70076"],"URL":"https:\/\/doi.org\/10.1111\/ped.70076","archive":["Portico"],"relation":{},"ISSN":["1328-8067","1442-200X"],"issn-type":[{"value":"1328-8067","type":"print"},{"value":"1442-200X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70076"}}