{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:59:16Z","timestamp":1790913556125,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T00:00:00Z","timestamp":1747699200000},"content-version":"vor","delay-in-days":139,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP21ck0106505h0003"],"award-info":[{"award-number":["JP21ck0106505h0003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP22ck0106759h0001"],"award-info":[{"award-number":["JP22ck0106759h0001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP23ck0106759h0002"],"award-info":[{"award-number":["JP23ck0106759h0002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["JP24ck0106759h0003"],"award-info":[{"award-number":["JP24ck0106759h0003"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007786","name":"National Center for Child Health and Development","doi-asserted-by":"publisher","award":["2021\u2010B4"],"award-info":[{"award-number":["2021\u2010B4"]}],"id":[{"id":"10.13039\/100007786","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007786","name":"National Center for Child Health and Development","doi-asserted-by":"publisher","award":["30S\u20102"],"award-info":[{"award-number":["30S\u20102"]}],"id":[{"id":"10.13039\/100007786","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatrics International"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>ZNF384<\/jats:italic>\n                      rearrangements (\n                      <jats:italic>ZNF384<\/jats:italic>\n                      \u2010r) are associated with distinct subgroups of B\u2010cell precursor acute lymphoblastic leukemia (BCP\u2010ALL) and the mixed phenotype of acute leukemia. Types of BCP\u2010ALL with\n                      <jats:italic>ZNF384<\/jats:italic>\n                      \u2010r exhibit common immunophenotypic characteristics, whereas their clinical features are not uniform and\n                      <jats:italic>TCF3::ZNF384<\/jats:italic>\n                      \u2010positive patients show a significantly poorer steroid response and higher frequency of relapse, while\n                      <jats:italic>EP300::ZNF384<\/jats:italic>\n                      \u2010positive patients exhibit a favorable response to conventional chemotherapy. Therefore, we aimed to investigate the differences in biological effects between these two ZNF384\u2010r molecules.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      We transduced BCP\u2010ALL cell lines with both\n                      <jats:italic>TCF3::ZNF384<\/jats:italic>\n                      and\n                      <jats:italic>EP300::ZNF384<\/jats:italic>\n                      by retrovirus\u2010mediated gene transduction, and examined the biological effects.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Flow cytometric analysis and RT\u2010qPCR revealed down\u2010regulation of CD10 in BCP\u2010ALL cells after transduction with both TCF3::ZNF384 and EP300::ZNF384. The annexin\u2010V binding apoptosis assay indicated that TCF3::ZNF384\u2010, but not EP300::ZNF384\u2010, expressing cells exhibited increased resistance to dexamethasone\u2010induced apoptosis. By means of an oligonucleotide microarray and RT\u2010qPCR, we observed that the transduction of\n                      <jats:italic>TCF3::ZNF384<\/jats:italic>\n                      , but not\n                      <jats:italic>EP300::ZNF384<\/jats:italic>\n                      , leads to significant enhancement of\n                      <jats:italic>cyclin D2<\/jats:italic>\n                      (\n                      <jats:italic>CCND2<\/jats:italic>\n                      ) gene expression in BCP\u2010ALL cells, but no growth advantage was observed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our data suggest that the acquisition of dexamethasone resistance in BCP\u2010ALL cell lines is an effect of TCF3::ZNF384 protein distinct from EP300::ZNF384. Other than the common functions of ZNF384\u2010r that contribute to the development of leukemia with a lineage\u2010ambiguous phenotype, TCF3::ZNF384 may exhibit a fusion partner\u2010dependent function distinct from EP300::ZNF384 and participate in the formation of characteristic clinical features of\n                      <jats:italic>TCF3<\/jats:italic>\n                      ::\n                      <jats:italic>ZNF384<\/jats:italic>\n                      \u2010expressing ALL patients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/ped.70078","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T07:09:51Z","timestamp":1747724991000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>TCF3<\/scp>\n                    ::\n                    <scp>ZNF384<\/scp>\n                    induces steroid resistance in B\u2010cell precursor acute lymphoblastic leukemia cells"],"prefix":"10.1111","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9949-1529","authenticated-orcid":false,"given":"Shinpei","family":"Kusano","sequence":"first","affiliation":[{"name":"Department of Pediatric Hematology and Oncology Research National Research Institute for Child Health and Development  Setagaya\u2010ku Japan"},{"name":"Department of Pediatrics and Adolescent Medicine Juntendo University Graduate School of Medicine  Bunkyo\u2010ku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitomi","family":"Ueno\u2010Yokohata","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology Research National Research Institute for Child Health and Development  Setagaya\u2010ku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Momoka","family":"Hori","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology Research National Research Institute for Child Health and Development  Setagaya\u2010ku Japan"},{"name":"Graduate School of Engineering Science Yokohama National University  Yokohama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Ishibashi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine Juntendo University Graduate School of Medicine  Bunkyo\u2010ku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junya","family":"Fujimura","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine Juntendo University Graduate School of Medicine  Bunkyo\u2010ku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0364-0022","authenticated-orcid":false,"given":"Toshiaki","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Adolescent Medicine Juntendo University Graduate School of Medicine  Bunkyo\u2010ku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Ohki","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology Research National Research Institute for Child Health and Development  Setagaya\u2010ku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9310-2126","authenticated-orcid":false,"given":"Nobutaka","family":"Kiyokawa","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology and Oncology Research National Research Institute for Child Health and Development  Setagaya\u2010ku Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,20]]},"reference":[{"key":"e_1_2_9_2_1","article-title":"Advances in B\u2010cell precursor acute lymphoblastic leukemia genomics","volume":"2","author":"Schwab C","year":"2018","journal-title":"Hema"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm10173792"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.111"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3535"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2016.04.038"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11790"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.26116"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.151035"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.209163.116"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375-021-01199-0"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0436-0"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2405084"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2012.753450"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-015-0197-2"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000448447"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3343\/alm.2016.36.4.396"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.28513"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.5.1649-1658.2000"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2001.16.1.10"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1158\/2643\u20103230.BCD\u201021\u20100163"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-33143-w"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.27891"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/267841a0"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-017-2220-6"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2015.11.009"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.10543"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-022-04135-6"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.1.114"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.13506"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1681-3"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41389-021-00377-0"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature22797"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-17-0912"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21072528"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33054"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-010-0110-1"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23063067"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-3625"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-45579-0"},{"key":"e_1_2_9_42_1","first-page":"5564","article-title":"Gene polymorphisms of SOCS1 and SOCS2 and acute lymphoblastic leukemia","volume":"24","author":"Chen SS","year":"2020","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2021.05.004"}],"container-title":["Pediatrics International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ped.70078","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/ped.70078","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/ped.70078","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:49:30Z","timestamp":1790912970000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/ped.70078"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":42,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/ped.70078"],"URL":"https:\/\/doi.org\/10.1111\/ped.70078","archive":["Portico"],"relation":{},"ISSN":["1328-8067","1442-200X"],"issn-type":[{"value":"1328-8067","type":"print"},{"value":"1442-200X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-05-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70078"}}