{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:16:41Z","timestamp":1760955401400},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,3,31]],"date-time":"2013-03-31T00:00:00Z","timestamp":1364688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Transplantation"],"published-print":{"date-parts":[[2013,8]]},"abstract":"Abstract<\/jats:title>There is limited evidence regarding the utility of circulating DSA<\/jats:styled-content> in surveillance for AMR<\/jats:styled-content> of pediatric heart recipients. Our hypothesis is that quantitation of DSA<\/jats:styled-content> improves their power for predicting a C<\/jats:styled-content>4d+, an integral component in the current diagnostic criteria of AMR<\/jats:styled-content>. All pediatric recipients transplanted between 10\/2005 and 1\/2011 were retrospectively reviewed for DSA<\/jats:styled-content> determined within 48\u00a0h of EMB<\/jats:styled-content>. C<\/jats:styled-content>4d+ was defined as >25% endothelial cell staining by immunohistochemical methods. A total of 183 paired DSA<\/jats:styled-content>\u2013EMB<\/jats:styled-content> determinations were identified in 60 patients, a median of three paired studies per patient (range: 1\u20139). DSA<\/jats:styled-content> were detected in 60 of these determinations. A receiver\u2010operating characteristic plot identified a threshold single\u2010antibody MFI<\/jats:styled-content> of >6000 that strongly correlated with C<\/jats:styled-content>4d+ (p\u00a0<\u00a00.0001) with a high negative predictive value (0.97) and specificity (0.95). The sensitivity and positive predictive values were 0.71 and 0.60, respectively. The predictive power of single\u2010antigen DSA<\/jats:styled-content> for C<\/jats:styled-content>4d deposition was improved in pediatric heart recipients using an institution\u2010specific MFI<\/jats:styled-content> threshold value. In post\u2010transplant care, quantitative DSA<\/jats:styled-content> should be an essential component in the surveillance for AMR<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/petr.12075","type":"journal-article","created":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T03:14:46Z","timestamp":1364786086000},"page":"429-435","source":"Crossref","is-referenced-by-count":15,"title":["Donor\u2010specific antibodies: Can they predict C4d deposition in pediatric heart recipients?"],"prefix":"10.1111","volume":"17","author":[{"given":"David M.","family":"Peng","sequence":"first","affiliation":[{"name":"University of Washington\/Seattle Children's Hospital Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuk M.","family":"Law","sequence":"additional","affiliation":[{"name":"University of Washington\/Seattle Children's Hospital Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariska S.","family":"Kemna","sequence":"additional","affiliation":[{"name":"University of Washington\/Seattle Children's Hospital Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Warner","sequence":"additional","affiliation":[{"name":"Puget Sound Blood Center Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Nelson","sequence":"additional","affiliation":[{"name":"Puget Sound Blood Center Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert J.","family":"Boucek","sequence":"additional","affiliation":[{"name":"University of Washington\/Seattle Children's Hospital Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,3,31]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2010.11.003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1053-2498(00)00095-4"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1053-2498(02)00472-2"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2006.08.009"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.tp.0000180564.14050.49"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2009.04.035"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.709022"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2009.01.015"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2007.09.011"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2005.08.014"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2011.07.012"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2007.07.011"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2010.03383.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0b013e3283352a50"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2003.08.028"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3046.2005.00425.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2009.10.016"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2005.03.019"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0803.2008.00176.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3046.2010.01435.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1017\/S1047951104006377","article-title":"Advances in methods for surveillance of rejection","volume":"1","author":"Boucek RJ","year":"2004","journal-title":"Cardiol Young"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2008.07.013"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2004.10.001"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/39.4.561"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-009-1386-4"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2009.06.009"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2004.07.021"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2008.07.005"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2010.06.019"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31822872dd"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2012.03.015"}],"container-title":["Pediatric Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpetr.12075","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/petr.12075","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T22:40:12Z","timestamp":1696200012000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/petr.12075"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,3,31]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1111\/petr.12075"],"URL":"https:\/\/doi.org\/10.1111\/petr.12075","archive":["Portico"],"relation":{},"ISSN":["1397-3142","1399-3046"],"issn-type":[{"value":"1397-3142","type":"print"},{"value":"1399-3046","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,3,31]]}}}