{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:21:30Z","timestamp":1767896490674,"version":"3.49.0"},"reference-count":15,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,6,14]],"date-time":"2014-06-14T00:00:00Z","timestamp":1402704000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Transplantation"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The ImmuKnow<jats:sup>\u00ae<\/jats:sup> <jats:styled-content style=\"fixed-case\">ICFA<\/jats:styled-content> reports <jats:italic>ex vivo<\/jats:italic> CD4 lymphocyte activation to quantify immunosuppression. Limited organ and age\u2010specific data exist for pediatric heart transplant recipients. We sought to examine their normative values and ICFA's association with rejection\/infection. A total of 380 ICFAs from 58 heart transplant recipients (6.5\/recipient) were studied retrospectively. The median age at the time of their first ICFA was 5.3\u00a0yr (IQR 2.4\u201312.1\u00a0yr). ICFA levels during immunologic stability (n\u00a0=\u00a0311) were a median of 305 (IQR: 172\u2013483) and mean of 353 (s.d. \u00b1 224) ng ATP\/mL. ICFA levels trended lower with advancing age. ICFA levels during immunologic stability increased over time from transplant after the first six\u00a0months but were not correlated with calcineurin inhibitor levels or the type used. There is no association between ICFA values during stability and rejection (median 368 ATP ng\/mL; IQR 153\u2013527) or infection (median 293 ATP ng\/mL; IQR 198\u2013432). In contrast to the manufacturer's suggested ranges, the immunologic stable ranges in pediatric cardiac recipients were very different. ICFA values during immunologic stability are related to time from transplant in pediatric heart recipients. ICFA's ability to discriminate rejection or infection from immunologic stability was not demonstrated.<\/jats:p>","DOI":"10.1111\/petr.12298","type":"journal-article","created":{"date-parts":[[2014,6,16]],"date-time":"2014-06-16T18:48:42Z","timestamp":1402944522000},"page":"485-490","source":"Crossref","is-referenced-by-count":5,"title":["Immune cell function assay in pediatric heart transplant recipients"],"prefix":"10.1111","volume":"18","author":[{"given":"Man\u2010Shun","family":"Wong","sequence":"first","affiliation":[{"name":"Department of Surgery Gold Coast Hospital Southport Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Boucek","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Seattle Children's Hospital University of Washington Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariska","family":"Kemna","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Seattle Children's Hospital University of Washington Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joe","family":"Rutledge","sequence":"additional","affiliation":[{"name":"Department of Pathology Seattle Children's Hospital University of Washington Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuk","family":"Law","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Seattle Children's Hospital University of Washington Seattle WA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,6,14]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-6196(12)65772-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/jlts.2001.29167"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199807150-00010"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199806113382407"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(00)00077-5"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200112270-00012"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2006.09.018"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2005.03.019"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2011.02.015"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.tp.0000234837.02126.70"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0012.2005.00429.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3046.2010.01321.x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2008.10.001"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2009.07.027"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.healun.2009.12.015"}],"container-title":["Pediatric Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpetr.12298","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/petr.12298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T23:11:20Z","timestamp":1695510680000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/petr.12298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,14]]},"references-count":15,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1111\/petr.12298"],"URL":"https:\/\/doi.org\/10.1111\/petr.12298","archive":["Portico"],"relation":{},"ISSN":["1397-3142","1399-3046"],"issn-type":[{"value":"1397-3142","type":"print"},{"value":"1399-3046","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,14]]}}}