{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T07:20:18Z","timestamp":1778570418352,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,12,26]],"date-time":"2014-12-26T00:00:00Z","timestamp":1419552000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Transplantation"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>In vivo<\/jats:italic> T\u2010cell depletion, using alemtuzumab therapy prior to <jats:styled-content style=\"fixed-case\">SCT<\/jats:styled-content>, can reduce the incidence of <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content>. This treatment has a potential to delay immune reconstitution resulting in increased morbidity due to viral illnesses. We retrospectively analyzed data on all pediatric patients with non\u2010malignant disorders who received alemtuzumab\u2010based conditioning regimens in our center over the last 10\u00a0yr (n\u00a0=\u00a091). Our data show an OS of 91.2%. The incidence of acute (grade 2\u20134) <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content> was 18.7% and that of chronic <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content> 5.5%. Viremia due to adenovirus, EBV and CMV was seen in 19.8%, 64.8% and 39.6% patients, respectively, with only two deaths attributed to viral infection (adenovirus). Chimerism level at three\u00a0month was predictive of graft outcome. Nine patients, who had graft failure after first <jats:styled-content style=\"fixed-case\">SCT<\/jats:styled-content>, were salvaged with a second <jats:styled-content style=\"fixed-case\">SCT<\/jats:styled-content> using RIC and same donor (if available). Based on these results, we conclude that the use of <jats:italic>in vivo<\/jats:italic> T\u2010cell depletion is safe, achieves good chimerism and does not lead to increased morbidity and mortality due to viral infections. It is associated with a reduced incidence of chronic <jats:styled-content style=\"fixed-case\">GVHD<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/petr.12416","type":"journal-article","created":{"date-parts":[[2014,12,26]],"date-time":"2014-12-26T05:24:09Z","timestamp":1419571449000},"page":"211-218","source":"Crossref","is-referenced-by-count":11,"title":["<i>In vivo<\/i> T\u2010cell depletion using alemtuzumab in family and unrelated donor transplantation for pediatric non\u2010malignant disease achieves engraftment with low incidence of graft vs. host disease"],"prefix":"10.1111","volume":"19","author":[{"given":"M. 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