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We also used NGS<\/jats:styled-content> to detect EBV DNA<\/jats:styled-content> within total genomic DNA<\/jats:styled-content>, and to evaluate its genetic variation. Sanger sequencing of major EBV<\/jats:styled-content> genes was used to compare SNV<\/jats:styled-content>s from samples taken from transplant patients vs. patients with IM<\/jats:styled-content>. We sequenced EBV DNA<\/jats:styled-content> from a healthy EBV<\/jats:styled-content>\u2010seropositive individual on a HiSeq 2000 instrument. Data were mapped to the EBV<\/jats:styled-content> reference genomes (AG<\/jats:styled-content>876 and B95\u20108). The number of EBNA<\/jats:styled-content>2 SNV<\/jats:styled-content>s was higher than for EBNA<\/jats:styled-content>1 and the other genes sequenced within comparable reference coordinates. For EBNA<\/jats:styled-content>2, there was a median of 15 SNV<\/jats:styled-content> among transplant samples compared with 10 among IM<\/jats:styled-content> samples (p = 0.036). EBNA<\/jats:styled-content>1 showed little variation between samples. For NGS<\/jats:styled-content>, we identified 640 and 892 variants at an unadjusted p value of 5 \u00d7 10\u22128<\/jats:sup> for AG<\/jats:styled-content>876 and B95\u20108 genomes, respectively. We used complementary sequence strategies to examine EBV<\/jats:styled-content> genetic diversity and its application to transplantation. The results provide the framework for further characterization of EBV<\/jats:styled-content> strains and related outcomes after organ transplantation.<\/jats:p>","DOI":"10.1111\/petr.12610","type":"journal-article","created":{"date-parts":[[2015,11,18]],"date-time":"2015-11-18T11:12:42Z","timestamp":1447845162000},"page":"124-129","source":"Crossref","is-referenced-by-count":8,"title":["The genetic diversity of Epstein\u2013Barr virus in the setting of transplantation relative to non\u2010transplant settings: A feasibility study"],"prefix":"10.1111","volume":"20","author":[{"given":"Upton D.","family":"Allen","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases Department of Paediatrics Hospital for Sick Children University of Toronto Toronto ON Canada"},{"name":"Research Institute Hospital for Sick Children University of Toronto Toronto ON Canada"},{"name":"The Transplant and Regenerative Medicine Centre Hospital for Sick Children University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pingzhao","family":"Hu","sequence":"additional","affiliation":[{"name":"The Centre for Applied Genomics Hospital for Sick Children Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio L.","family":"Pereira","sequence":"additional","affiliation":[{"name":"The Centre for Applied Genomics Hospital for Sick Children Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan L.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Paediatrics Stollery Children's Hospital Edmonton AB Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara A.","family":"Paton","sequence":"additional","affiliation":[{"name":"The Centre for Applied Genomics Hospital for Sick Children Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Beyene","sequence":"additional","affiliation":[{"name":"Research Institute Hospital for Sick Children University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasser","family":"Khodai\u2010Booran","sequence":"additional","affiliation":[{"name":"Research Institute Hospital for Sick Children University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Dipchand","sequence":"additional","affiliation":[{"name":"The Transplant and Regenerative Medicine Centre Hospital for Sick Children University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"H\u00e9bert","sequence":"additional","affiliation":[{"name":"The Transplant and Regenerative Medicine Centre Hospital for Sick Children University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicky","family":"Ng","sequence":"additional","affiliation":[{"name":"The Transplant and Regenerative Medicine Centre Hospital for Sick Children University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Nalpathamkalam","sequence":"additional","affiliation":[{"name":"The Transplant and Regenerative Medicine Centre Hospital for Sick Children University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stanley","family":"Read","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases Department of Paediatrics Hospital for Sick Children University of Toronto Toronto ON Canada"},{"name":"Research Institute Hospital for Sick Children University of Toronto Toronto ON Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,11,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.12004"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.5.1346"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3046.2005.00318.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(64)91524-7"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1542\/peds.75.6.1003","article-title":"Epstein\u2010Barr virus infectious mononucleosis in children. 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