{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T15:01:26Z","timestamp":1761490886706,"version":"3.41.2"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,20]],"date-time":"2019-10-20T00:00:00Z","timestamp":1571529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Transplantation"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Autologous hematopoietic stem cell transplantation (aHSCT) using hematopoietic progenitor cells (HPCs) has become an important therapeutic modality for patients with high\u2010risk malignancies. Current literature on standardized method for HPC apheresis in children is sparse and failure rate reported as high as 30%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Patients\/Methods<\/jats:title><jats:p>A retrospective study of 125 pediatric patients with high\u2010risk malignancies undergoing aHSCT in Western Australia between 1997 and 2016 was conducted.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Mobilization was achieved by means of chemotherapy and granulocyte colony\u2010stimulating factor (G\u2010CSF). Patients underwent apheresis the day after CD34<jats:sup>+<\/jats:sup> counts reached \u226520\/\u00b5L and an additional dose of G\u2010CSF. Peripheral arterial and intravenous lines were inserted in pediatric intensive care unit under local anesthetic and\/or sedation, omitting the need for general anesthesia as well as facilitating an uninterrupted apheresis flow. Larger apheresis total blood volumes were processed in patients weighing \u226420\u00a0kg. The minimal dose of \u22652\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> CD34<jats:sup>+<\/jats:sup> cells\/kg was successfully collected in 98.4% of all patients. The optimal dose of 3\u20105\u00a0\u00d7\u00a010<jats:sup>6<\/jats:sup> CD34<jats:sup>+<\/jats:sup> cells\/kg was collected in 96% of patients scheduled for a single aHSCT, 87.5% for tandem, and 100% for triple aHSCT. All HPC collections were completed in one apheresis session. Mobilization after \u22643 chemotherapy cycles and cycles including cyclophosphamide resulted in a significantly higher yield of CD34<jats:sup>+<\/jats:sup> cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our approach to HPC mobilization by means of chemotherapy and single myeloid growth factor combined with optimal collection timing facilitated by continuous apheresis flow resulted in highly effective HPC harvest in children and adolescents with high\u2010risk cancers.<\/jats:p><\/jats:sec>","DOI":"10.1111\/petr.13602","type":"journal-article","created":{"date-parts":[[2019,10,21]],"date-time":"2019-10-21T06:25:02Z","timestamp":1571639102000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Optimized peripheral blood progenitor cell mobilization for autologous hematopoietic cell transplantation in children with high\u2010risk and refractory malignancies"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0254-7486","authenticated-orcid":false,"given":"Eliska","family":"Furlong","sequence":"first","affiliation":[{"name":"Department of Paediatric and Adolescent Haematology, Oncology, Blood and Marrow Transplantation Perth Children\u2019s Hospital Perth WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jesper","family":"Jensen","sequence":"additional","affiliation":[{"name":"PathWest Laboratory Medicine WA Perth WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Woodard","sequence":"additional","affiliation":[{"name":"Paediatric Intensive Care Unit Perth Children\u2019s Hospital Perth WA Australia"},{"name":"School of Nursing, Midwifery and Paramedicine Curtin University Perth WA 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Children\u2019s Hospital Perth WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hazel","family":"Gough","sequence":"additional","affiliation":[{"name":"Department of Paediatric and Adolescent Haematology, Oncology, Blood and Marrow Transplantation Perth Children\u2019s Hospital Perth WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natasha","family":"Bear","sequence":"additional","affiliation":[{"name":"Department of Clinical Research and Education Perth Children\u2019s Hospital Perth WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tina L.","family":"Carter","sequence":"additional","affiliation":[{"name":"Department of Paediatric and Adolescent Haematology, Oncology, Blood and Marrow Transplantation Perth Children\u2019s Hospital Perth WA Australia"},{"name":"PathWest Laboratory Medicine WA Perth WA Australia"},{"name":"Division of Paediatrics School of Medicine University of Western Australia Perth 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