{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T07:08:04Z","timestamp":1767337684298,"version":"3.41.2"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,12,11]],"date-time":"2019-12-11T00:00:00Z","timestamp":1576022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["5R01HL119747"],"award-info":[{"award-number":["5R01HL119747"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Transplantation"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Heart transplantation is a well\u2010established therapy for end\u2010stage heart failure in children and young adults. The highest risk of graft loss occurs in the first 60\u00a0days post\u2010transplant. Donor fraction of cell\u2010free DNA is a highly sensitive marker of graft injury. Changes in cell\u2010free DNA levels have not previously been studied in depth in patients early after heart transplant. A prospective study was conducted among heart transplant recipients at a single pediatric heart center. Blood samples were collected from children and young adult transplant patients at three time points within 10\u00a0days of transplantation. DF and total cell\u2010free DNA levels were measured using a targeted method (myTAI<jats:sub>HEART<\/jats:sub>). In 17 patients with serial post\u2010transplant samples, DF peaks in the first 2\u00a0days after transplant (3.5%, [1.9\u201010]%) and then declines toward baseline (0.27%, [0.19\u20100.52]%) by 6\u20109\u00a0days. There were 4 deaths in the first year among the 10 patients with complete sample sets, and 3 out of 4 who died had a late rise or blunted decline in donor fraction. Patients who died trended toward an elevated total cell\u2010free DNA at 1\u00a0week (41.5, [34\u201065] vs 13.6, [6.2\u201022] <jats:italic>P<\/jats:italic>\u00a0=\u00a0.07). Donor fraction peaks early after heart transplant and then declines toward baseline. Patients without sustained decline in donor fraction and\/or elevated total cell\u2010free DNA at 1\u00a0week may have worse outcomes.<\/jats:p>","DOI":"10.1111\/petr.13622","type":"journal-article","created":{"date-parts":[[2019,12,11]],"date-time":"2019-12-11T11:25:24Z","timestamp":1576063524000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Early changes in cell\u2010free DNA levels in newly transplanted heart transplant patients"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1838-4714","authenticated-orcid":false,"given":"Steven D.","family":"Zangwill","sequence":"first","affiliation":[{"name":"Division of Cardiology Phoenix Children's Hospital Phoenix AZ USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven J.","family":"Kindel","sequence":"additional","affiliation":[{"name":"Division of Pediatric Cardiology Medical College of Wisconsin Herma Heart Institute, Children's Wisconsin Milwaukee WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William S.","family":"Ragalie","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery David Geffen School of Medicine at UCLA Los Angeles CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula E.","family":"North","sequence":"additional","affiliation":[{"name":"Department of Pathology Medical College of Wisconsin Children's Hospital of Wisconsin Wauwatosa WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alyssa","family":"Pollow","sequence":"additional","affiliation":[{"name":"Division of Pediatric Cardiology Medical College of Wisconsin Herma Heart Institute, Children's Wisconsin Milwaukee WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mats","family":"Hidestrand","sequence":"additional","affiliation":[{"name":"Division of Pediatric Cardiothoracic Surgery, Medical College of Wisconsin Herma Heart Institute, Children's Wisconsin Milwaukee WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aoy","family":"Tomita\u2010Mitchell","sequence":"additional","affiliation":[{"name":"Division of Pediatric Cardiothoracic Surgery, Medical College of Wisconsin Herma Heart Institute, Children's Wisconsin Milwaukee WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karl D.","family":"Stamm","sequence":"additional","affiliation":[{"name":"Tai Diagnostics Inc. Wauwatosa WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael E.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Division of Pediatric Cardiothoracic Surgery, Medical College of Wisconsin Herma Heart Institute, Children's Wisconsin Milwaukee WI USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,12,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2007.01.093"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)79055-3"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2013.09.029"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1013924108"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3007803"},{"key":"e_1_2_9_7_1","first-page":"14(4)","article-title":"Graft\u2010derived cell free DNA, a non\u2010invasive early rejection and graft damage marker in liver transplantation: a prospective observational, multicenter cohort study","volume":"25","author":"Schutz E","year":"2017","journal-title":"PLoS Med"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2017.10.029"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/306580"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-016-1578-9"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2018.04.026"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1159\/000335020"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbi.2008.08.010"}],"container-title":["Pediatric Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpetr.13622","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/petr.13622","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/petr.13622","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/petr.13622","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T08:11:08Z","timestamp":1694160668000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/petr.13622"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,11]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,2]]}},"alternative-id":["10.1111\/petr.13622"],"URL":"https:\/\/doi.org\/10.1111\/petr.13622","archive":["Portico"],"relation":{},"ISSN":["1397-3142","1399-3046"],"issn-type":[{"type":"print","value":"1397-3142"},{"type":"electronic","value":"1399-3046"}],"subject":[],"published":{"date-parts":[[2019,12,11]]},"assertion":[{"value":"2019-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13622"}}