{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T11:47:02Z","timestamp":1780055222066,"version":"3.54.0"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2019\/23270\u20100"],"award-info":[{"award-number":["2019\/23270\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Pediatric Transplantation"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Intestinal failure in children, when unresponsive to rehabilitation, requires intestinal transplantation as the only definitive therapy. In regions with limited availability of deceased donors, living\u2010donor intestinal transplantation (LDIT) represents an important alternative. The early immunometabolic consequences of venous drainage configuration, however, remain insufficiently defined. Because ischemia\u2013reperfusion injury is central to graft dysfunction, understanding how portal versus systemic venous outflow shapes the immediate postoperative response is essential to guide pediatric strategies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A juvenile swine model (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200914) was used to compare portal (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20097) and systemic (caval,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20097) venous drainage after LDIT. Animals were followed for 4\u2009days with serial biochemical, histological, immunohistochemical, and molecular assessments. Analyses included linear mixed\u2010effects models (LMM) for repeated measures and principal component analysis (PCA) to integrate multivariable data and identify global immunometabolic patterns.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Hepatic and renal function were preserved in both groups. Histology revealed only mild ischemia\u2013reperfusion injury (Chiu\/Park grades 1\u20132), with a trend toward greater lymphocytic infiltration in the systemic group. Caspase immunohistochemistry demonstrated early apoptotic activation in the portal group, which declined by day 4, suggesting a controlled adaptive response. IL\u20101\u03b1 expression was selectively upregulated in intestinal tissue from the portal group, consistent with early mucosal immune activation. PCA confirmed a distinct immunometabolic profile under portal drainage, characterized by balanced inflammation, controlled apoptosis, and trends toward enhanced protein synthesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Venous drainage configuration modulates early biological responses after LDIT. Portal drainage was associated with a more regulated immunometabolic profile, supporting the hypothesis that physiological venous outflow promotes mucosal protection and immune balance.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/petr.70210","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T04:49:21Z","timestamp":1761281361000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Portal Venous Drainage Modulates Inflammatory and Apoptotic Responses in a Swine Model of Living Donor Intestinal Transplantation"],"prefix":"10.1111","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1437-249X","authenticated-orcid":false,"given":"Guilherme F.","family":"Paganoti","sequence":"first","affiliation":[{"name":"Institute for Children and Adolescents at the University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3855-3298","authenticated-orcid":false,"given":"Uenis","family":"Tannuri","sequence":"additional","affiliation":[{"name":"Institute for Children and Adolescents at the University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2153-5146","authenticated-orcid":false,"given":"Alessandro R.","family":"Belon","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5402-6455","authenticated-orcid":false,"given":"Josiane O.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5853-5743","authenticated-orcid":false,"given":"Suellen","family":"Serafini","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raimundo R. N.","family":"Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Institute for Children and Adolescents at the University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5106-7497","authenticated-orcid":false,"given":"Felipe Y.","family":"Matsushita","sequence":"additional","affiliation":[{"name":"University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5481-032X","authenticated-orcid":false,"given":"Ana Cristina A.","family":"Tannuri","sequence":"additional","affiliation":[{"name":"Institute for Children and Adolescents at the University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1602650"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/jpen.2232"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2016.04.009"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPG.0000000000001722"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu16172966"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu15102341"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cld.2018.12.009"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0000000000003065"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2003.50139"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.17150"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rcl.2023.04.008"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1072-7515(02)01482-5"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-1345(02)02717-3"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2006.02.152"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00423-010-0727-x"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-1345(03)00455-X"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/antib8040052"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1446072"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1970.01340280030009"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1996.v110.pm8964408"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jss.2018.01.007"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11756-007-0098-0"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19980101)31:1<1::AID-CYTO1>3.0.CO;2-R"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surg.2004.06.008"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.021908.132612"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-200301000-00014"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.170.9.4759"}],"container-title":["Pediatric Transplantation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/petr.70210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T09:37:16Z","timestamp":1762940236000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/petr.70210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,23]]},"references-count":27,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1111\/petr.70210"],"URL":"https:\/\/doi.org\/10.1111\/petr.70210","archive":["Portico"],"relation":{},"ISSN":["1397-3142","1399-3046"],"issn-type":[{"value":"1397-3142","type":"print"},{"value":"1399-3046","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,23]]},"assertion":[{"value":"2025-07-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70210"}}