{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T07:04:40Z","timestamp":1791011080925,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,11,19]],"date-time":"2012-11-19T00:00:00Z","timestamp":1353283200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photochem &amp; Photobiology"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Listeria monocytogenes<\/jats:italic>, a food\u2010borne bacterial pathogen causing significant human mortality, propagates by expressing genes in response to environmental signals, such as temperature and pH. <jats:italic>Listeria<\/jats:italic> gene (<jats:italic>lmo0799<\/jats:italic>) encodes a protein homologous to the <jats:italic>Bacillus subtilis<\/jats:italic> YtvA, which has a flavin\u2010light, oxygen or voltage (LOV) domain and a Sulfate Transporters Anti\u2010Sigma factor antagonist (STAS) output domain that regulates transcription\u2010initiation factor Sigma B in the bacterial stress response upon exposure to light. This could be significant for the pathogenesis of listeriosis because Sigma B has been linked to virulence of <jats:italic>Listeria<\/jats:italic>, and the <jats:italic>Listeria<\/jats:italic> Lmo0799 protein has recently been identified as a virulence factor activated by blue light. We have cloned, expressed heterologously in <jats:italic>Escherichia coli<\/jats:italic> and purified the full\u2010length LM\u2010LOV\u2010STAS protein. Although it exhibits photochemical activity similar to that of YtvA, LM\u2010LOV\u2010STAS lacks an almost universally conserved arginine in the flavin\u2010binding site, as well as another positively charged residue, a lysine in YtvA. The absence of these positive charges was found to destabilize retention of the flavin mononucleotide (FMN) chromophore in the LM\u2010LOV\u2010STAS protein, particularly at higher temperatures. The unusual sequence of the LM\u2010LOV\u2010STAS protein alters both spectral features and activation\/deactivation kinetics, potentially expanding the sensory capacity of this LOV domain, <jats:italic>e.g<\/jats:italic>. to detect light plus cold.<\/jats:p>","DOI":"10.1111\/php.12004","type":"journal-article","created":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T17:51:32Z","timestamp":1349113892000},"page":"361-369","source":"Crossref","is-referenced-by-count":23,"title":["Photocycle of the <scp>LOV<\/scp>\u2010<scp>STAS<\/scp> Protein from the Pathogen <i>Listeria monocytogenes<\/i>."],"prefix":"10.1111","volume":"89","author":[{"given":"Ruby H.","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry University of California Santa Cruz CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of California Santa Cruz CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto A.","family":"Bogomolni","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry University of California Santa Cruz CA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,11,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00716-06"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"3650","DOI":"10.1128\/JB.180.14.3650-3656.1998","article-title":"General stress transcription factor sigma(B) and its role in acid tolerance and virulence of Listeria monocytogenes","volume":"180","author":"Wiedmann M.","year":"1998","journal-title":"J. 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