{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T09:13:13Z","timestamp":1778663593498,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,7,8]],"date-time":"2013-07-08T00:00:00Z","timestamp":1373241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photochem &amp; Photobiology"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study evaluated physical, chemical and morphological changes in lungs of mice infected with <jats:italic>Paracoccidioides brasiliensis<\/jats:italic>. The animals were inoculated with 0.1\u00a0mL of fungal suspension of the <jats:italic>P. brasiliensis<\/jats:italic> 18 isolate and were euthanized 1, 2, 4 and 8\u00a0weeks after inoculation. The upper left lobe of the lung was isolated, fixed and processed for paraffin embedding. The sections were stained with H&amp;E for histopathological study, with Gomori\u2010Grocott to locate and identify the fungus, and with TUNEL immunostaining to detect the occurrence of programmed cell death. The lower and middle right lobes were analyzed by Fourier Transform Infrared Photoacoustic Spectrocopy (FTIR\u2010PAS) to investigate physical and chemical features of the infected lungs. The results showed that lungs infected by <jats:italic>P. brasiliensis<\/jats:italic> underwent structural changes that varied according to the time period analyzed, and that changes in the absorption bands of different chemical groups resulted from these morphological changes. The results suggest that the combination of FTIR\u2010PAS spectroscopy with morphological evaluation is an effective procedure for the study of paracoccidioidomycosis, one of the most important systemic mycoses that can damage the lung architecture and consequently impair the respiratory function.<\/jats:p>","DOI":"10.1111\/php.12110","type":"journal-article","created":{"date-parts":[[2013,6,7]],"date-time":"2013-06-07T05:17:18Z","timestamp":1370582238000},"page":"1170-1175","source":"Crossref","is-referenced-by-count":14,"title":["Morphological and Structural Changes in Lung Tissue Infected by <i>Paracoccidioides brasiliensis<\/i>:<scp> FTIR<\/scp> Photoacoustic Spectroscopy and Histological Analysis"],"prefix":"10.1111","volume":"89","author":[{"given":"Edilaine M.","family":"Morato","sequence":"first","affiliation":[{"name":"Department of Morphological Science Universidade Estadual de Maring\u00e1 Maring\u00e1 PR Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gutierrez R.","family":"Morais","sequence":"additional","affiliation":[{"name":"Department of Physics Universidade Estadual de Maring\u00e1 Maring\u00e1 PR Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francielle","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Physics Universidade Estadual de Maring\u00e1 Maring\u00e1 PR Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio N.","family":"Medina","sequence":"additional","affiliation":[{"name":"Department of Physics Universidade Estadual de Maring\u00e1 Maring\u00e1 PR Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terezinha I. 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