{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T12:56:33Z","timestamp":1774788993926,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,2,23]],"date-time":"2014-02-23T00:00:00Z","timestamp":1393113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photochem &amp; Photobiology"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Xeroderma pigmentosum (XP) is a genetic disorder associated with defects in nucleotide excision repair, which eliminates a wide variety of helix\u2010distorting types of <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage including sunlight\u2010induced pyrimidine dimers. In addition to skin disease, approximately 30% of <jats:styled-content style=\"fixed-case\">XP<\/jats:styled-content> patients develop progressive neurological disease, which has been hypothesized to be associated with the accumulation of a particular type of oxidatively generated <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage called purine 8,5\u2032\u2010cyclo\u20102\u2032\u2010deoxynucleosides (purine cyclonucleosides). However, there are no currently available methods to detect purine cyclonucleosides in <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> without the need for <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> hydrolysis. In this study, we generated a novel monoclonal antibody (CdA\u20101) specific for purine cyclonucleosides in single\u2010stranded <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> that recognizes 8,5\u2032\u2010cyclo\u20102\u2032\u2010deoxyadenosine (cyclo\u2010<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content>). An immunoassay using CdA\u20101 revealed a linear dose response between known amounts of cyclo\u2010<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content> in oligonucleotides and the antibody binding to them. The quantitative immunoassay revealed that treatment with Fenton\u2010type reagents (CuCl<jats:sub>2<\/jats:sub>\/H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\/ascorbate) efficiently produces cyclo\u2010<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content> in DNA in a dose\u2010dependent manner. Moreover, immunofluorescent analysis using CdA\u20101 enabled the visualization of cyclo\u2010<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content> in human osteosarcoma cells, which had been transfected with oligonucleotides containing cyclo\u2010<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content>. Thus, the CdA\u20101 antibody is a valuable tool for the detection and quantification of cyclo\u2010<jats:styled-content style=\"fixed-case\">dA<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>, and may be useful for characterizing the mechanism(s) underlying the development of XP neurological disease.<\/jats:p>","DOI":"10.1111\/php.12239","type":"journal-article","created":{"date-parts":[[2014,1,29]],"date-time":"2014-01-29T08:48:47Z","timestamp":1390985327000},"page":"829-836","source":"Crossref","is-referenced-by-count":12,"title":["Quantitative and <i>in situ<\/i> Detection of Oxidatively Generated <scp>DNA<\/scp> Damage 8,5\u2032\u2010Cyclo\u20102\u2032\u2010Deoxyadenosine Using an Immunoassay with a Novel Monoclonal Antibody"],"prefix":"10.1111","volume":"90","author":[{"given":"Takaaki","family":"Iwamoto","sequence":"first","affiliation":[{"name":"Radioisotope Research Center Nara Medical University School of Medicine Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip J.","family":"Brooks","sequence":"additional","affiliation":[{"name":"Laboratory of Neurogenetics National Institute on Alcohol Abuse and Alcoholism Bethesda MD"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohisa","family":"Nishiwaki","sequence":"additional","affiliation":[{"name":"Radioisotope Research Center Nara Medical University School of Medicine Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuki","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Radioisotope Research Center Nara Medical University School of Medicine Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhiko","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Dermatology Nara Medical University School of Medicine Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeki","family":"Sugiura","sequence":"additional","affiliation":[{"name":"Medical Genetics Research Center Nara Medical University School of Medicine Kashihara Nara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshio","family":"Mori","sequence":"additional","affiliation":[{"name":"Radioisotope Research Center Nara Medical University School of Medicine Kashihara Nara 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