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However, the use of this antibiotic is often connected with the risk of phototoxic reactions that lead to various skin disorders. One of the factors influencing the photosensitivity reactions is the melanin content in melanocytes. In this study, the impact of doxycycline and <jats:styled-content style=\"fixed-case\">UVA<\/jats:styled-content> irradiation on cell viability, melanogenesis and antioxidant defense system in cultured normal human epidermal melanocytes (<jats:styled-content style=\"fixed-case\">HEM<\/jats:styled-content>n\u2010<jats:styled-content style=\"fixed-case\">DP<\/jats:styled-content>) was examined. The exposure of cells to doxycycline and <jats:styled-content style=\"fixed-case\">UVA<\/jats:styled-content> radiation resulted in concentration\u2010dependent loss in melanocytes viability and induced melanin biosynthesis. Significant changes were stated in cellular antioxidant enzymes activity: <jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> CAT<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GP<\/jats:styled-content>x, which indicates alterations of antioxidant defense system. The results obtained <jats:italic>in\u00a0vitro<\/jats:italic> may explain the mechanisms of phototoxic reactions that occur in normal human epidermal melanocytes <jats:italic>in\u00a0vivo<\/jats:italic> after exposure of skin to doxycycline and <jats:styled-content style=\"fixed-case\">UVA<\/jats:styled-content> radiation.<\/jats:p>","DOI":"10.1111\/php.12497","type":"journal-article","created":{"date-parts":[[2015,7,18]],"date-time":"2015-07-18T09:12:51Z","timestamp":1437210771000},"page":"1429-1434","source":"Crossref","is-referenced-by-count":26,"title":["Modulation of Melanogenesis and Antioxidant Status of Melanocytes in Response to Phototoxic Action of Doxycycline"],"prefix":"10.1111","volume":"91","author":[{"given":"Jakub","family":"Rok","sequence":"first","affiliation":[{"name":"Chair and Department of Pharmaceutical Chemistry School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec Medical University of Silesia Sosnowiec Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Buszman","sequence":"additional","affiliation":[{"name":"Chair and Department of Pharmaceutical Chemistry School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec Medical University of Silesia Sosnowiec Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Artur","family":"Beberok","sequence":"additional","affiliation":[{"name":"Chair and Department of Pharmaceutical Chemistry School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec Medical University of Silesia Sosnowiec Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcin","family":"Delijewski","sequence":"additional","affiliation":[{"name":"Chair and Department of Pharmaceutical Chemistry School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec Medical University of Silesia Sosnowiec Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micha\u0142","family":"Otr\u0119ba","sequence":"additional","affiliation":[{"name":"Chair and Department of Pharmaceutical Chemistry School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec Medical University of Silesia Sosnowiec Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorota","family":"Wrze\u015bniok","sequence":"additional","affiliation":[{"name":"Chair and Department of Pharmaceutical Chemistry School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec Medical University of Silesia Sosnowiec Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,8,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.2.232-260.2001"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00216-6"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/367622"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1139\/O08-002"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2005.10.004"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/138955712798868977"},{"key":"e_1_2_6_8_1","first-page":"247","article-title":"The effect of doxycycline on atherogenesis in apoE\u2010knockout mice","volume":"62","author":"Pawlowska M.","year":"2011","journal-title":"J. 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