{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T18:45:10Z","timestamp":1787942710280,"version":"build-2784847793"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T00:00:00Z","timestamp":1487721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010174","name":"University of Colorado","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010174","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photochem &amp; Photobiology"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Whether ascorbate oxidation is promoted by <jats:styled-content style=\"fixed-case\">UVA<\/jats:styled-content> light in human lenses and whether this process is influenced by age and <jats:styled-content style=\"fixed-case\">GSH<\/jats:styled-content> levels are not known. In this study, we used paired lenses from human donors. One lens of each pair was exposed to <jats:styled-content style=\"fixed-case\">UVA<\/jats:styled-content> light, whereas the other lens was kept in the dark for the same period of time as the control. Using <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">MS<\/jats:styled-content> analyses, we found that older lenses (41\u201373 years) were more susceptible to <jats:styled-content style=\"fixed-case\">UVA<\/jats:styled-content>\u2010induced ascorbate oxidation than younger lenses (18\u201340 years). Approximately 36% of the ascorbate (relative to control) was oxidized in older lenses compared to ~16% in younger lenses. Furthermore, lenses with higher levels of <jats:styled-content style=\"fixed-case\">GSH<\/jats:styled-content> were less susceptible to <jats:styled-content style=\"fixed-case\">UVA<\/jats:styled-content>\u2010induced ascorbate oxidation compared to those with lower levels, and this effect was not dependent on age. The oxidation of ascorbate led to elevated levels of reactive <jats:italic>\u03b1<\/jats:italic>\u2010dicarbonyl compounds. In summary, our study showed that <jats:styled-content style=\"fixed-case\">UVA<\/jats:styled-content> light exposure leads to ascorbate oxidation in human lenses and that such oxidation is more pronounced in aged lenses and is inversely related to <jats:styled-content style=\"fixed-case\">GSH<\/jats:styled-content> levels. Our findings suggest that <jats:styled-content style=\"fixed-case\">UVA<\/jats:styled-content> light exposure could lead to protein aggregation through ascorbate oxidation in human lenses.<\/jats:p>","DOI":"10.1111\/php.12717","type":"journal-article","created":{"date-parts":[[2017,1,13]],"date-time":"2017-01-13T15:22:40Z","timestamp":1484320960000},"page":"1091-1095","source":"Crossref","is-referenced-by-count":5,"title":["<scp>UVA<\/scp> Light\u2010mediated Ascorbate Oxidation in Human Lenses"],"prefix":"10.1111","volume":"93","author":[{"given":"Stefan","family":"Rakete","sequence":"first","affiliation":[{"name":"Department of Ophthalmology School of Medicine University of Colorado Aurora CO"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram H.","family":"Nagaraj","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology School of Medicine University of Colorado Aurora CO"},{"name":"Department of Pharmaceutical Sciences Skaggs School of Pharmacy and Pharmaceutical Sciences University of Colorado Aurora CO"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.78.6.478"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a112404"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01203684"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1996.tb05669.x"},{"key":"e_1_2_6_6_1","first-page":"2165","article-title":"UV absorbance of the human cornea in the 240\u2010 to 400\u2010nm range","volume":"43","author":"Kolozsv\u00e1ri L.","year":"2002","journal-title":"Invest. 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