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As a result the spleen and other lymphoid organs are targets of paracoccidioidomycosis. There are few studies describing this disease in the spleen and more detailed descriptions are required. This study combines Histopathology and Fourier Transform Raman Spectroscopy (<jats:styled-content style=\"fixed-case\">FT<\/jats:styled-content>\u2010Raman) methods to study spleen infected by Pb. The Swiss mice were euthanized after 1, 2, 4 and 8 weeks of infection with Pb, and their spleens were removed for <jats:italic>ex vivo<\/jats:italic> analyzes. Histopathological evaluation revealed that the red pulp was the area most affected, presenting the highest concentration of yeasts, inflammatory cells and cells in apoptosis, with no observation of typical granuloma formation. The main physical\u2013chemical changes were detected in the spectral ranges between 1730\u20131590 cm<jats:sup>\u22121<\/jats:sup> and 1390\u20131280 cm<jats:sup>\u22121<\/jats:sup> attributed to amide I and vibrational deformation mode of <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub> and <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>2<\/jats:sub> molecules, respectively. The amide I was correlated to total protein content and <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>3<\/jats:sub> and <jats:styled-content style=\"fixed-case\">CH<\/jats:styled-content><jats:sub>2<\/jats:sub> molecules to collagen of the spleen. The association between histological and physical\u2013chemical methods enabled detection of several alterations in the spleen, including apoptosis, contributing to a better understanding of paracoccidioidomycosis, a tropical neglected disease.<\/jats:p>","DOI":"10.1111\/php.12840","type":"journal-article","created":{"date-parts":[[2017,9,7]],"date-time":"2017-09-07T19:54:49Z","timestamp":1504814089000},"page":"88-94","source":"Crossref","is-referenced-by-count":6,"title":["Combination of Histopathology and FT\u2010Raman Spectroscopy for the Study of Experimental Paracoccidioidomycosis in the Spleen"],"prefix":"10.1111","volume":"94","author":[{"given":"Elaine Sciuniti Benites","family":"Mansano","sequence":"first","affiliation":[{"name":"Department of Morphological Sciences Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gutierrez Rodrigu\u00eas","family":"de Morais","sequence":"additional","affiliation":[{"name":"Department of Physics Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edilaine Martins","family":"Moratto","sequence":"additional","affiliation":[{"name":"Department of Morphological Sciences Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francielle","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Physics Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro Luciano","family":"Baesso","sequence":"additional","affiliation":[{"name":"Department of Physics Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Neto","family":"Medina","sequence":"additional","affiliation":[{"name":"Department of Physics Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terezinha Inez Estivalet","family":"Svidzinski","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis and Biomedicine Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luzmarina","family":"Hernandes","sequence":"additional","affiliation":[{"name":"Department of Morphological Sciences Universidade Estadual de Maring\u00e1 Maring\u00e1 Paran\u00e1 Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"1245","DOI":"10.1590\/S1806-37132009001200013","article-title":"Chapter 6\u2010paracoccidioidomycosis","volume":"35","author":"Wanke B.","year":"2009","journal-title":"J. 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