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XP\u2010V cellular phenotypes may be aggravated if proteins of DNA damage response (DDR) pathway are blocked, as widely demonstrated by experiments with UVC light and caffeine. However, little is known about the participation of DDR in XP\u2010V cells exposed to UVA light, the wavelengths patients are mostly exposed. Here, we demonstrate the participation of ATR kinase in protecting XP\u2010V cells after receiving low UVA doses using a specific inhibitor, with a remarkable increase in sensitivity and \u03b3H2AX signaling. Corroborating ATR participation in UVA\u2010DDR, a significant increase in Chk1 protein phosphorylation, as well as S\u2010phase cell cycle arrest, is also observed. Moreover, the participation of oxidative stress is supported by the antioxidant action of <jats:italic>N<\/jats:italic>\u2010acetylcysteine (NAC), which significantly protects XP\u2010V cells from UVA light, even in the presence of the ATR inhibitor. These findings indicate that the ATR\/Chk1 pathway is activated to control UVA\u2010induced oxidatively generated DNA damage and emphasizes the role of ATR kinase as a mediator of genomic stability in pol eta defective cells.<\/jats:p>","DOI":"10.1111\/php.13041","type":"journal-article","created":{"date-parts":[[2018,10,26]],"date-time":"2018-10-26T07:04:53Z","timestamp":1540537493000},"page":"345-354","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["ATR\/Chk1 Pathway is Activated by Oxidative Stress in Response to UVA Light in Human Xeroderma Pigmentosum Variant Cells"],"prefix":"10.1111","volume":"95","author":[{"given":"Nat\u00e1lia Cestari","family":"Moreno","sequence":"first","affiliation":[{"name":"Department of Microbiology Institute of Biomedical Sciences University of Sao Paulo Sao Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camila Carri\u00e3o Machado","family":"Garcia","sequence":"additional","affiliation":[{"name":"NUPEB &amp; Biological Sciences Department Federal University of Ouro Preto Ouro Preto MG Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clarissa Ribeiro Reily","family":"Rocha","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Biomedical Sciences University of Sao Paulo Sao Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veridiana","family":"Munford","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Biomedical Sciences University of Sao Paulo Sao Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos Frederico Martins","family":"Menck","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Biomedical Sciences University of Sao Paulo Sao Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200200523"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2003.11.001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.01.029"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.60.8.0004"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(01)00206-8"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0781.2008.00319.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C1PP05219E"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/1011-1344(90)85014-N"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1991.tb02034.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/B810085C"},{"key":"e_1_2_7_12_1","doi-asserted-by":"crossref","first-page":"26732","DOI":"10.1016\/S0021-9258(19)61437-7","article-title":"Distribution and repair of bipyrimidine photoproducts in solar UV\u2010irradiated mammalian cells: Possible role of Dewar photoproducts in solar mutagenesis","volume":"275","author":"Perdiz D.","year":"2000","journal-title":"J. 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