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Previous studies showed that these bacteria were susceptible to blue light phototoxicity mediated by the production of reactive oxygen species (<jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>). In the present study, we tested the effect of blue light on the integrity <jats:italic>Fusobacterium nucleatum<\/jats:italic>'s membrane, cellular proteins and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. Bacterial samples were exposed to high intensity blue light for 0, 70, 140 and 280\u00a0s (<jats:italic>i.e<\/jats:italic>. fluences of 0, 96, 192 and 384\u00a0J\u00a0cm<jats:sup>\u22122<\/jats:sup>, respectively). Following light exposure, bacterial samples were examined for membrane damage using fluorescence microscopy, intra\u2010cellular protein analysis using electrophoresis (<jats:styled-content style=\"fixed-case\">SDS<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PAGE<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> fragmentation using ultra\u2013filtration. Results showed that the increasing exposure of bacterial samples to blue light caused increased membrane permeability concomitant with a reduction in intra\u2010cellular proteins and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> fragments content. These results suggest that membrane damage is the main effect of high intensity blue light exposure on malodour producing bacteria.<\/jats:p>","DOI":"10.1111\/php.13151","type":"journal-article","created":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T11:59:04Z","timestamp":1565697544000},"page":"178-181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Effect of High Intensity Blue Light on <i>Fusobacterium nucleatum<\/i> Membrane Integrity"],"prefix":"10.1111","volume":"96","author":[{"given":"Uziel","family":"Jeffet","sequence":"first","affiliation":[{"name":"Department of Prosthodontics Goldschleger School of Dental Medicine Tel Aviv University Tel Aviv Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Shimon","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics Goldschleger School of Dental Medicine Tel Aviv University Tel Aviv 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