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Currently, topical PDT is an effective treatment for several skin diseases, including bacterial infections, fungal mycoses and psoriasis. In addition, PDT is also used to treat nonmelanoma skin cancer and can be a potential tool for melanoma, associated with other treatments. In this work, we evaluated the antitumor photoactivity of a new pyrene\u2010based PS (TPPy) by using the murine melanoma cell line (B16F10). The <jats:italic>in vitro<\/jats:italic> permeation\/retention tests in porcine ear skin were also performed in order to evaluate the potential application of the PS for topical use in skin cancer. Moreover, to determine the toxicity <jats:italic>in vivo<\/jats:italic>, we used the <jats:italic>Galleria mellonella<\/jats:italic> as an alternative animal model of study. The results showed that TPPy is a promising PS for application in PDT, with potential antitumor photoactivity (IC<jats:sub>50<\/jats:sub> 6.5\u00a0\u03bcmol L<jats:sup>\u20101<\/jats:sup>), absence of toxicity in the <jats:italic>G. mellonella<\/jats:italic> model at higher concentration (70.0\u00a0mmol L<jats:sup>\u22121<\/jats:sup>) and the accumulation tendency in the epidermis plus dermis sites (165.20\u00a0\u00b1\u00a04.12\u00a0ng\u00a0cm<jats:sup>\u22122<\/jats:sup>).<\/jats:p>","DOI":"10.1111\/php.13335","type":"journal-article","created":{"date-parts":[[2020,9,24]],"date-time":"2020-09-24T01:53:35Z","timestamp":1600912415000},"page":"408-415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>In vitro<\/i> Studies of Antitumor Effect, Toxicity\/Cytotoxicity and Skin Permeation\/Retention of a Green Fluorescence Pyrene\u2010based Dye for PDT 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