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In this study, we aimed to evaluate the effects of near\u2010infrared low\u2010intensity laser PBM on proliferation, viability and osteogenic differentiation of stem cells isolated from human periodontal ligament. A 940\u2010nm diode laser with an energy density of 4\u00a0J\u00a0cm<jats:sup>\u22122<\/jats:sup> in a 100\u2010mW continuous wave was used for irradiation in 3 sessions every 48h. Cell viability was measured 24, 48 and 72\u00a0h after irradiation. The effects of laser on mineralized tissue deposition were evaluated by using Alizarin red staining after dividing cells into three groups of nonosteogenic medium (C\u2212), an osteogenic medium without laser (C+), and an osteogenic medium with laser irradiation (L+). Gene expression levels were also evaluated by real\u2010time PCR. Our results showed no significant difference between MTT levels of the study and control groups. After 14 and 21\u00a0days, both L+ and C+ groups showed an increase in mineralized tissue formation compared to the C\u2212 group. There was an increase in VEGF and BMP expressions compared to C\u2212. In conclusion, the irradiation setting used in this study may be able to improve mineralized tissue deposition.<\/jats:p>","DOI":"10.1111\/php.13367","type":"journal-article","created":{"date-parts":[[2020,12,11]],"date-time":"2020-12-11T05:30:00Z","timestamp":1607664600000},"page":"851-859","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["The Effect of Laser Photobiomodulation on Periodontal Ligament Stem Cells"],"prefix":"10.1111","volume":"97","author":[{"given":"Leila","family":"Gholami","sequence":"first","affiliation":[{"name":"Department of Periodontics School of Dentistry Hamadan University of Medical Sciences Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilda","family":"Parsamanesh","sequence":"additional","affiliation":[{"name":"Basic and Molecular Epidemiology of Gastrointestinal Disorder Research Center Research Institute for Gastroenterology and Liver Diseases Shahid 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