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To understand the impact of these hydroperoxides in complex biological systems, methods allowing their characterization and accurate quantification must be available. In this work, hydroperoxides derived from tryptophan and tyrosine and from peptides containing these amino acids were synthesized by photooxidation, and characterized by high\u2010resolution mass spectrometry. In addition, experiments were carried out to compare two colorimetric methods commonly used for quantification of peroxides, namely the iodometric and the ferric\u2010xylenol orange assays. For the tryptophan hydroperoxide, the quantifications obtained by colorimetric methods were then compared to that obtained by NMR. The results showed that for the ferric\u2010xylenol orange method, the stoichiometry between peroxide and Fe<jats:sup>3+<\/jats:sup> ions varies considerably. On the other hand, for the iodometric assay, the stoichiometry peroxide:I<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> ions is always 1:1. However, the kinetics of the reactions of peroxides with I<jats:sup>\u2212<\/jats:sup> vary, and the assay must be performed in anaerobic conditions. Thus, the iodometric method is more appropriate for precise quantification of a given peroxide. The characterization and accurate quantification of biological peroxides is\u00a0key to understand the mechanisms involved in redox processes.<\/jats:p>","DOI":"10.1111\/php.13623","type":"journal-article","created":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T19:55:12Z","timestamp":1648842912000},"page":"678-686","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Characterization and Quantification of Tryptophan\u2010 and Tyrosine\u2010derived Hydroperoxides<sup>\u2020<\/sup>"],"prefix":"10.1111","volume":"98","author":[{"given":"Stella Boutris","family":"Jayme","sequence":"first","affiliation":[{"name":"Department of Biochemistry Institute of Chemistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4458-4354","authenticated-orcid":false,"given":"Fernanda Manso","family":"Prado","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Chemistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Pereira","family":"Massafera","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Chemistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2340-6770","authenticated-orcid":false,"given":"Graziella Eliza","family":"Ronsein","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Chemistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4125-8350","authenticated-orcid":false,"given":"Paolo","family":"Di Mascio","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Institute of Chemistry University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.85"},{"key":"e_1_2_5_3_1","first-page":"1","article-title":"Defining roles of specific reactive oxygen species (ROS) in cell biology and physiology","author":"Sies H.","year":"2022","journal-title":"Nat. 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