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Tissue engineering using mesenchymal stem cells (MSCs) is a promising method to manage such injuries. Moreover, photobiomodulation therapy (PBMT) can enhance this treatment. The present study aimed to investigate the effect of PBMT on differentiation of MSCs derived from dental follicle (DF) into neurons. MSCs were isolated from an impacted tooth follicle by digestion method. The stem cells were cultured, and differentiated into neurons. The cells received two sessions of PBMT with 810 or 980\u2009nm diode laser (100 mW, 4 J\u2009cm<jats:sup>\u22122<\/jats:sup>) in either DMEM or neural inductive medium. Phenotypic characterization of the cells was determined using flow cytometry. In addition, \u03b2\u2010tubulin and MAP2 genes expression level changes were analyzed using RT\u2010PCR and western blot technique. After 14 days, flow cytometry analysis confirmed the mesenchymal nature of cells. RT\u2010PCR and western blot affirmed the expression of \u03b2\u2010tubulin and MAP2 genes and proteins respectively. PBMT with both wavelengths significantly increased \u03b2\u2010tubulin and MAP2 expression in neural inductive medium with highest expression mean in 980 nm group. PBMT with 810 and 980 nm lasers could be a promising adjunctive method in differentiation of DF\u2010originated MSCs into neural cells.<\/jats:p>","DOI":"10.1111\/php.13627","type":"journal-article","created":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T19:59:05Z","timestamp":1648843145000},"page":"1434-1440","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Effect of Photobiomodulation Therapy on Differentiation of Mesenchymal Stem Cells Derived from Impacted Third Molar Tooth into Neuron\u2010like Cells"],"prefix":"10.1111","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5840-1988","authenticated-orcid":false,"given":"Farnoush","family":"Mohammadi","sequence":"first","affiliation":[{"name":"Craniomaxillofacial Research Center Tehran University of Medical Sciences  Tehran Iran"},{"name":"Oral and Maxillofacial Surgery Department School of Dentistry Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3575-4094","authenticated-orcid":false,"given":"Naghmeh","family":"Bahrami","sequence":"additional","affiliation":[{"name":"Craniomaxillofacial Research Center Tehran University of Medical Sciences  Tehran Iran"},{"name":"Department of Tissue Engineering School of Advanced Technologies in Medicine Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahya","family":"Nazariyan","sequence":"additional","affiliation":[{"name":"School of Dentistry Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdolreza","family":"Mohamadnia","sequence":"additional","affiliation":[{"name":"Chronic Respiratory Diseases Research Center National Research Institute of Tuberculosis and Lung Diseases (NRITLD) Shahid Beheshti University of Medical Sciences  Tehran Iran"},{"name":"Department of Biotechnology School of Advanced Technologies in Medicine Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5188-0325","authenticated-orcid":false,"given":"Neda","family":"Hakimiha","sequence":"additional","affiliation":[{"name":"Laser Application in Medical Sciences Research Center Shahid Beheshti University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abolfazl","family":"Nazariyan","sequence":"additional","affiliation":[{"name":"Clinical biochemistry Department Faculty of Medicine Zanjan University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,4,20]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"34","article-title":"Nerve damage in dentistry","volume":"65","author":"Pogrel M. 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