{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T16:03:53Z","timestamp":1782230633634,"version":"3.54.5"},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,5,25]],"date-time":"2022-05-25T00:00:00Z","timestamp":1653436800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004742","name":"National Academy of Sciences of Ukraine","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004742","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Photochem &amp; Photobiology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Caged compounds comprise the group of artificially synthesized, light\u2010sensitive molecules that enable <jats:italic>in\u00a0situ<\/jats:italic> derivation of biologically active constituents capable of affecting cells, tissues and\/or biological processes upon exposure to light. Ruthenium\u2010bispyridine (RuBi) complexes are photolyzed by biologically harmless visible light. In the present study, we show that RuBi\u2010caged nicotine can be used as a source of free nicotine to induce proliferation of A549 nonsmall\u2010cell lung cancer (NSCLC) cells by acting on nicotinic acetylcholine receptors expressed in these cells. RuBi\u2010nicotine was photolyzed using LED light source with the spectrum matching RuBi\u2010absorption. Photorelease of free nicotine ([Nic]<jats:sub>p\/r<\/jats:sub>) was quantified by high\u2010performance liquid chromatography (HPLC). 5\u2010s\u2010long light exposure of 10\u2009\u03bc<jats:sc>m<\/jats:sc> of RuBi\u2010nicotine generated 2\u2009\u03bc<jats:sc>m<\/jats:sc> [Nic]<jats:sub>p\/r<\/jats:sub> which enhanced A549 cell proliferation similarly to the 2\u2009\u03bc<jats:sc>m<\/jats:sc> of plain nicotine during 72\u2009h of cell culturing. Both RuBi\u2010nicotine <jats:italic>per se<\/jats:italic> and its photolysis byproduct exerted no effect on A549 cells. We conclude that RuBi\u2010nicotine can be a good source of free nicotine for inducing short\u2010 and long\u2010term biological effects. Photolysis of RuBi\u2010nicotine is quite effective, and can produce biologically relevant concentrations of nicotine at acceptable concentrations of the source material with the use of simple, inexpensive, and easily accessible light sources.<\/jats:p>","DOI":"10.1111\/php.13652","type":"journal-article","created":{"date-parts":[[2022,5,15]],"date-time":"2022-05-15T14:38:30Z","timestamp":1652625510000},"page":"78-82","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Induction of <scp>A549<\/scp> Nonsmall\u2010Cell Lung Cancer Cells Proliferation by Photoreleased Nicotine"],"prefix":"10.1111","volume":"99","author":[{"given":"Danylo I.","family":"Kravchuk","sequence":"first","affiliation":[{"name":"Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ganna V.","family":"Sotkis","sequence":"additional","affiliation":[{"name":"Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mykola M.","family":"Shcherbatiuk","sequence":"additional","affiliation":[{"name":"Kholodny Institute of Botany of the National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruslan M.","family":"Kravchuk","sequence":"additional","affiliation":[{"name":"Institute of Physics of the National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vassili G.","family":"Nazarenko","sequence":"additional","affiliation":[{"name":"Institute of Physics of the National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petro P.","family":"Gorbyk","sequence":"additional","affiliation":[{"name":"Chuiko Institute of Surface Chemistry of the National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1451-7188","authenticated-orcid":false,"given":"Yaroslav M.","family":"Shuba","sequence":"additional","affiliation":[{"name":"Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine  Kyiv Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth1072"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsta.2012.0330"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinorgbio.2010.08.003"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.7b00083"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0278943"},{"key":"e_1_2_6_7_1","unstructured":"A549 Cell Line Origin(2022). Available at:http:\/\/www.a549.com\/. Accessed on 30 March 2022."},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1998.4172"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2007.12.006"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-13-0541"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3725"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/bph.13954"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00573"},{"key":"e_1_2_6_14_1","volume-title":"A Language and Environment for Statistical Computing","author":"R Core Team","year":"2021"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.21105\/joss.03167"},{"key":"e_1_2_6_16_1","unstructured":"Wickham H.L.&HenryL(2020) Tidyr: Tidy Messy Data R Package Version 1.0.2. Available at:https:\/\/CRAN.R\u2010project.org\/package=tidyr.Accessed on 30 March 2022."},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.280.6219.972"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4637"}],"container-title":["Photochemistry and Photobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/php.13652","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/php.13652","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/php.13652","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T07:56:58Z","timestamp":1692518218000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/php.13652"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,25]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2023,1]]}},"alternative-id":["10.1111\/php.13652"],"URL":"https:\/\/doi.org\/10.1111\/php.13652","archive":["Portico"],"relation":{},"ISSN":["0031-8655","1751-1097"],"issn-type":[{"value":"0031-8655","type":"print"},{"value":"1751-1097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,25]]},"assertion":[{"value":"2022-03-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}