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However, the specific role of PBM in odontogenic differentiation of SHEDs is little know, and we further investigated potential mechanism of PBM osteo\/odontogenisis. A 980\u2009nm diode laser with different energy densities of (0.5, 5, 10\u2009J\u2009cm<jats:sup>\u22122<\/jats:sup>) in a 100\u2010mW continuous wave was used for irradiation every 24\u2009h. Osteo\/odontogenic differentiation of SHEDs was achieved by performing alkaline phosphatase (ALP) and alizarin red staining (ARS) and osteo\/odontogenic markers were also evaluated by qRT\u2010PCR and western blotting. Additionally, western blot and immunohistochemical staining were performed to evaluate the levels of BMP\/Smad and Wnt\/\u03b2\u2010catenin signaling\u2010related proteins. We found that PBM at 5\u2009J\u2009cm<jats:sup>\u22121<\/jats:sup> increased mineral deposition and upregulated the expression of related osteo\/odontogenic markers along with the elevated expression of \u03b2\u2010catenin and phosphorylation level of Smad1\/5\/9. Furthermore, Wnt signaling inhibition using DKK1 and BMP signaling inhibition using noggin inhibited PBM\u2010induced osteo\/odontogenic marker expression when used individually or jointly. In conclusion, PBM induces the osteo\/odontogenic differentiation of SHEDs through cross talk between BMP\/Smad and Wnt\/\u03b2\u2010catenin signaling pathways.<\/jats:p>","DOI":"10.1111\/php.13751","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T06:46:20Z","timestamp":1669617980000},"page":"1181-1192","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["980\u2009nm Photobiomodulation Promotes Osteo\/Odontogenic Differentiation of the Stem Cells from Human Exfoliated Deciduous Teeth <i>Via<\/i> the Cross\u00a0talk Between <scp>BMP<\/scp>\/Smad and Wnt\/\u03b2\u2010Catenin Signaling Pathways"],"prefix":"10.1111","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0292-287X","authenticated-orcid":false,"given":"Ziting","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry, Shenzhen Stomatology Hospital (Pingshan) Southern Medical 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