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Some photophysical properties were performed in ethanol and phosphate buffer, and the type of aggregates form in phosphate buffer was analyzed. Using the 1,3\u2010diphenylisobenzofuran assay, a qualitative assessment of the capacity of dyes to produce singlet oxygen after irradiation was performed. Regarding the antifungal activity, this was studied through a broth microdilution assay using\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    PYCC 4072 as a biological model. The effect of irradiation of the dyes, with an appropriate light emitting diode system, on the antifungal activity was also evaluated, and it was verified that some of the dyes improve their activity after irradiation. Using fluorescence microscopy techniques, the colocalization of dyes in\n                    <jats:italic>S. cerevisae<\/jats:italic>\n                    cells was investigated and it was possible to verify that some of the squarylium dyes with a barbituric moiety in the four\u2010membered central ring stained and accumulated preferentially in the mitochondrial web and perinuclear membrane of the cells. The possible use as a fluorescent probe for the detection of HSA was also evaluated for one of the dyes of the series, demonstrating a linear variation in the fluorescence intensity accompanied by the increase in the protein concentration.\n                  <\/jats:p>","DOI":"10.1111\/php.13762","type":"journal-article","created":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T03:44:25Z","timestamp":1671162265000},"page":"1282-1292","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of Antifungal Activity and Potential Application as Fluorescent Probes of Indolenine and Benzo[\n                    <i>e<\/i>\n                    ]\n                    <scp>Indole\u2010Based<\/scp>\n                    Squarylium Dyes"],"prefix":"10.1111","volume":"99","author":[{"given":"Vanessa S. D.","family":"Gomes","sequence":"first","affiliation":[{"name":"Department of Chemistry, Centre of Chemistry \u2013 Vila Real (CQ\u2010VR) University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro  Vila\u00a0Real Portugal"},{"name":"Department of Chemistry, Centre of Chemistry (CQ\u2010UM) University of Minho  Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o C. C.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Centre of Chemistry (CQ\u2010UM) University of Minho  Braga Portugal"},{"name":"Department of Biology, Centre of Molecular and Environmental Biology (CBMA) University of Minho  Braga Portugal"},{"name":"Institute of Science and Innovation for Bio\u2010Sustainability (IBS) University of Minho  Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato E.","family":"Boto","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Health Sciences Research Centre (CICS\u2010UBI) University of Beira Interior  Covilh\u00e3 Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo","family":"Almeida","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Health Sciences Research Centre (CICS\u2010UBI) University of Beira Interior  Covilh\u00e3 Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 R.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Physical Department, Centre of Chemistry \u2013 Vila Real (CQ\u2010VR) University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro  Vila Real Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Jo\u00e3o","family":"Sousa","sequence":"additional","affiliation":[{"name":"Department of Biology, Centre of Molecular and Environmental Biology (CBMA) University of Minho  Braga Portugal"},{"name":"Institute of Science and Innovation for Bio\u2010Sustainability (IBS) University of Minho  Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5654-9359","authenticated-orcid":false,"given":"Lucinda V.","family":"Reis","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Centre of Chemistry \u2013 Vila Real (CQ\u2010VR) University of Tr\u00e1s\u2010os\u2010Montes and Alto Douro  Vila\u00a0Real Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2048-1190","authenticated-orcid":false,"given":"M. Sameiro T.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Centre of Chemistry (CQ\u2010UM) University of Minho  Braga Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,1,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-534-7_22"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.1997.tb01078.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.4084\/mjhid.2011.001"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00202"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00120"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ph14121312"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.11.019"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/adfm.202008201"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/sym14050966"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dyepig.2013.06.022"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1533\/9780857093974.2.588"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2021.114071"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.bioconjchem.9b00482"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotochemrev.2017.03.001"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1010-6030(03)00203-X"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s43630-022-00175-6"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2017.08.010"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dyepig.2019.108024"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.4155\/fmc-2022-0112"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1562\/0031-8655(2002)0760672SDFPTS2.0.CO2"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867322666150319120134"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dyepig.2019.04.007"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotochem.2020.112710"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2021.105221"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.13624"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2860(03)00155-8"},{"key":"e_1_2_6_28_1","volume-title":"Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard\u2014Third Edition","author":"CLSI","year":"2008"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12039-007-0013-9"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00581"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/8135608"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlumin.2011.05.048"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0010-8545(02)00034-6"},{"key":"e_1_2_6_34_1","unstructured":"Calculation of molecular properties and drug\u2010likeness\u2010Molinspiration cheminformatics software tool. 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