{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T08:32:53Z","timestamp":1777537973521,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T00:00:00Z","timestamp":1687737600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV\u201020\u20100340"],"award-info":[{"award-number":["APVV\u201020\u20100340"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","award":["APVV\u201021\u20100333"],"award-info":[{"award-number":["APVV\u201021\u20100333"]}],"id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["ITMS2014+: 313011AUW6"],"award-info":[{"award-number":["ITMS2014+: 313011AUW6"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["ITMS2014+: 313011V455"],"award-info":[{"award-number":["ITMS2014+: 313011V455"]}],"id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Photochem &amp; Photobiology"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Time\u2010resolved phosphorescence detection was employed to determine the lifetime of singlet oxygen in live cells. Using hypericin as a photosensitizer, singlet oxygen was generated in U87MG glioblastoma cells. The phosphorescence of singlet oxygen was detected in aqueous cell suspensions following pulsed laser excitation. Our goal was to eliminate or reduce the problems associated with lifetime measurements in water\u2010based cell suspensions. The apparatus enabled simultaneous singlet oxygen phosphorescence and transient absorption measurements, reducing uncertainty in lifetime estimation. The changes in singlet oxygen lifetime were observed during early and late apoptosis induced by photodynamic action. Our findings show that the effective lifetime of singlet oxygen in the intracellular space of the studied glioblastoma cells is 0.4\u2009\u03bcs and increases to 1.5\u2009\u03bcs as apoptosis progresses. Another group of hypericin, presumably located in the membrane blebs and the plasma membrane of apoptotic cells, generates singlet oxygen with a lifetime of 1.9\u2009\u03bcs.<\/jats:p>","DOI":"10.1111\/php.13828","type":"journal-article","created":{"date-parts":[[2023,6,26]],"date-time":"2023-06-26T10:11:11Z","timestamp":1687774271000},"page":"159-171","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Singlet oxygen lifetime changes in dying glioblastoma cells"],"prefix":"10.1111","volume":"100","author":[{"given":"Andrej","family":"Hovan","sequence":"first","affiliation":[{"name":"Department of Biophysics, Faculty of Science P.J. \u0160af\u00e1rik University in Ko\u0161ice  Ko\u0161ice Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viktoria","family":"Pevna","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Faculty of Science P.J. \u0160af\u00e1rik University in Ko\u0161ice  Ko\u0161ice Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronika","family":"Huntosova","sequence":"additional","affiliation":[{"name":"Center for Interdisciplinary Biosciences, Technology and Innovation Park P.J. \u0160af\u00e1rik University in Ko\u0161ice  Ko\u0161ice Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pavol","family":"Miskovsky","sequence":"additional","affiliation":[{"name":"Cassovia New Industry Cluster  Ko\u0161ice Slovak Republic"},{"name":"SAFTRA Photonics Ltd.  Ko\u0161ice Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8807-2118","authenticated-orcid":false,"given":"Gregor","family":"B\u00e1n\u00f3","sequence":"additional","affiliation":[{"name":"Department of Biophysics, Faculty of Science P.J. \u0160af\u00e1rik University in Ko\u0161ice  Ko\u0161ice Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201600055"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2008.00359.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja2010708"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/b307576c"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(01)00207-X"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1562\/2006-06-09-IR-914"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0378660"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1562\/0031-8655(2002)0750382DNILDO2.0.CO2"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1039\/b804333g"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp206739y"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/1011-1344(90)85006-I"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1991.tb03669.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1992.tb04273.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1562\/2006-04-10-IR-868"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/b609070m"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp051163i"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja055342p"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ar040075y"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2009.11.013"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/lapl.200810116"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp311629a"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2pp05409d"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2018.12.013"},{"key":"e_1_2_7_25_1","first-page":"2141","article-title":"Spheroidal model of SKBR3 and U87MG cancer cells for live imaging of Caspase\u20103 during apoptosis induced by singlet oxygen in photodynamic therapy","volume":"10","author":"Pevna V","year":"2022","journal-title":"Biomedicine"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2010.01.010"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp109661c"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2018.07.009"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2016.02.042"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp3114539"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2017.01.019"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/b809049a"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1021\/jp984030o"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/j100138a015"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201600127"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00233-7"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.8b00659"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2020.119173"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1562\/0031-8655(2002)0760320POHBTH2.0.CO2"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchem.120"}],"container-title":["Photochemistry and Photobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/php.13828","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,21]],"date-time":"2024-01-21T05:24:31Z","timestamp":1705814671000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/php.13828"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,6,26]]},"references-count":39,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1]]}},"alternative-id":["10.1111\/php.13828"],"URL":"https:\/\/doi.org\/10.1111\/php.13828","archive":["Portico"],"relation":{},"ISSN":["0031-8655","1751-1097"],"issn-type":[{"value":"0031-8655","type":"print"},{"value":"1751-1097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,6,26]]},"assertion":[{"value":"2023-05-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-06-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}