{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T13:41:09Z","timestamp":1737034869442,"version":"3.33.0"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T00:00:00Z","timestamp":1713398400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Photochem &amp; Photobiology"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Excessive exposure to blue light can cause retinal damage. Hydrogen\u2010rich saline (HRS), one of the hydrogen therapies, has been demonstrated to be effective in eye photodamage, but the effect on the expression of melanopsin in intrinsically photosensitive retinal ganglion cells (ipRGCs) is unknown. In this study, we used a rat model of light\u2010induced retinal injury to observe the expression of melanopsin after HRS treatment and to determine the effect of HRS on retinal ganglion cell protection. Adult SD rats were exposed to blue light (48\u2009h) and treated with HRS for 0, 3, 7, and 14\u2009days. Real\u2010time polymerase chain reaction (qRT\u2010PCR) and Western blotting (WB) were performed to find the expression of genes and proteins, respectively. The function of retinal ipRGCs was measured by pattern\u2010evoked electroretinography (pERG). The number and morphological changes of melanopsin\u2010positive ganglion cells in the retina were observed by immunofluorescence (IF). Acute blue light exposure caused a decrease in ipRGC function, decreased expression of melanopsin protein and the melanopsin\u2010positive RGCs, and diminished immunoreactivity in dendrites. However, over time, melanopsin showed a tendency to self\u2010recovery, with an increase in melanopsin protein expression and the number of melanopsin\u2010positive RGCs, with incomplete recovery of function within two weeks. HRS treatment accelerated the recovery process, with a significant increase in melanopsin expression and the number of melanopsin\u2010positive RGCs, and an improvement in the pERG waveform within two weeks.<\/jats:p>","DOI":"10.1111\/php.13952","type":"journal-article","created":{"date-parts":[[2024,4,18]],"date-time":"2024-04-18T10:00:55Z","timestamp":1713434455000},"page":"106-115","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effect of hydrogen\u2010rich saline on melanopsin after acute blue light\u2010induced retinal damage in rats"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4639-6771","authenticated-orcid":false,"given":"Xiao","family":"Wang","sequence":"first","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Vision Science  Tianjin P. R. China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University  Tianjin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifan","family":"Sun","sequence":"additional","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Vision Science  Tianjin P. R. China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University  Tianjin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changlin","family":"Luan","sequence":"additional","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Vision Science  Tianjin P. R. China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University  Tianjin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shiqiao","family":"Yang","sequence":"additional","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Vision Science  Tianjin P. R. China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University  Tianjin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kailei","family":"Wang","sequence":"additional","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Vision Science  Tianjin P. R. China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University  Tianjin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoran","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Vision Science  Tianjin P. R. China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University  Tianjin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Hao","sequence":"additional","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Vision Science  Tianjin P. R. China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University  Tianjin P. R. China"},{"name":"Nankai University Eye Institute, Nankai University Affiliated Eye Hospital Nankai University  Tianjin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Tianjin Eye Hospital Tianjin Key Lab of Ophthalmology and Vision Science  Tianjin P. R. China"},{"name":"Clinical College of Ophthalmology Tianjin Medical University  Tianjin P. R. China"},{"name":"Nankai University Eye Institute, Nankai University Affiliated Eye Hospital Nankai University  Tianjin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.12250"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2020.109547"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.survophthal.2006.06.009"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01413.2009"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03345"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06829"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.2012.01120.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn768"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.212910"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.120.6.791"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.10652"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/6435364"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep21018"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1577"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/cns.12258"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-010-9317-6"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.15-17034"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.63.1.26"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-021-02380-3"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22136732"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/3236892"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-28254-8"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.12484"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/php.13244"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/ejn.15659"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.3233\/JPD-202178"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-3214"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2012.11.006"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2014.08.012"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-023-01353-w"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.4062\/biomolther.2021.155"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu14214451"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2017.05.010"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.08.055"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1069609"}],"container-title":["Photochemistry and Photobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/php.13952","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T13:14:10Z","timestamp":1737033250000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/php.13952"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,18]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1111\/php.13952"],"URL":"https:\/\/doi.org\/10.1111\/php.13952","archive":["Portico"],"relation":{},"ISSN":["0031-8655","1751-1097"],"issn-type":[{"type":"print","value":"0031-8655"},{"type":"electronic","value":"1751-1097"}],"subject":[],"published":{"date-parts":[[2024,4,18]]},"assertion":[{"value":"2023-12-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-04-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}