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It is, therefore, important to understand the effects of ascites, including the associated fluid shear stress (FSS), on phenotypic changes and therapy response, specifically FSS\u2010induced chemotherapy resistance and the underlying mechanisms in ovarian cancer. This study investigated the effects of FSS on response to cisplatin, a platinum\u2010based chemotherapy, and doxorubicin, an anthracycline, both of which are commonly used to manage advanced\u2010stage ovarian cancer. Consistent with prior research, OVCAR\u20103 and Caov\u20103 cells cultivated under FSS demonstrated significant resistance to cisplatin. Examination of the role of mitochondria revealed an increase in mitochondrial DNA copy number and intracellular ATP content in cultures grown under FSS, suggesting that changes in mitochondria number and metabolic activity may contribute to platinum resistance. Interestingly, no resistance to doxorubicin was observed under FSS, the first such observation of a lack of resistance under these conditions. Finally, this study demonstrated the potential of photodynamic priming using benzoporphyrin derivative, a clinically approved photosensitizer that localizes in part to mitochondria and endoplasmic reticula, to enhance the efficacy of cisplatin, but not doxorubicin, thereby overcoming FSS\u2010induced platinum resistance.<\/jats:p>","DOI":"10.1111\/php.13967","type":"journal-article","created":{"date-parts":[[2024,6,8]],"date-time":"2024-06-08T03:30:27Z","timestamp":1717817427000},"page":"1676-1693","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Overcoming the effects of fluid shear stress in ovarian cancer cell lines: Doxorubicin alone or photodynamic priming to target platinum resistance"],"prefix":"10.1111","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8203-0427","authenticated-orcid":false,"given":"Marta","family":"Overchuk","sequence":"first","affiliation":[{"name":"Joint Department of 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