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Zebrafish embryos were divided into three groups: Control (<jats:italic>n<\/jats:italic>\u2009=\u200960), Green (G) (<jats:italic>n<\/jats:italic>\u2009=\u200960), and Red (R) (<jats:italic>n<\/jats:italic>\u2009=\u200960), then irradiated for three days (14\u2009hours\/day) with 0.5 W\/cm<jats:sup>2<\/jats:sup> G (\u03bbpeak\u2009=\u2009520\u2009nm, 180\u2009mA) and R (\u03bbpeak\u2009=\u2009660\u2009nm, 210\u2009mA). At the end of 72\u2009h, pancreatic and beta cells, circadian rhythm, and oxidative stress gene were analyzed using RT\u2010PCR. Malondialdehyde, nitric oxide, superoxide dismutase, and glutathione levels were also evaluated. In the Red group, pancreatic area increased by ~97.13% compared to the Control group and by approximately ~62.16% compared to the G group (both <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001), and no significant difference in beta cell area (<jats:italic>p<\/jats:italic>\u2009=\u20090.964). G group insulin expression increased 2.31\u2010fold compared to R group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). Red LED treatment increased MDA levels (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), oxidative stress (<jats:italic>fth1b<\/jats:italic>, <jats:italic>nqo1<\/jats:italic>) (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001), and <jats:italic>per1b<\/jats:italic> during the photophase (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) compared to G group. R LED treatment increases oxidative stress and disrupts circadian rhythm, leading to reduced insulin secretion. The positive effects of G LED treatment have potential for metabolic syndrome, diabetes, and pancreatic diseases.<\/jats:p>","DOI":"10.1111\/php.14050","type":"journal-article","created":{"date-parts":[[2024,12,10]],"date-time":"2024-12-10T04:53:55Z","timestamp":1733806435000},"page":"1327-1342","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["520\u2009nm and 660\u2009nm light\u2010emitting diodes modulates pancreatic development and beta cell functions in zebrafish embryos"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0804-1475","authenticated-orcid":false,"given":"\u00dcnsal Veli","family":"\u00dcst\u00fcnda\u011f","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Istanbul Atlas University  Istanbul Turkey"},{"name":"Department of Biochemistry, Institute of Health Sciences Marmara University  Istanbul 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