{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T17:12:00Z","timestamp":1780506720103,"version":"3.54.1"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["465763\/2014\u20106"],"award-info":[{"award-number":["465763\/2014\u20106"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010749","name":"Comiss\u00e3o Nacional de Energia Nuclear","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010749","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Photochem &amp;amp; Photobiology"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Interest in antimicrobial photodynamic therapy for treating cutaneous leishmaniasis has been rising, showing promising outcomes and good patient tolerance. In this study, we aimed to develop a protocol for producing giant plasma membrane vesicles (GPMVs) from\n                    <jats:italic>Leishmania amazonensis<\/jats:italic>\n                    promastigote cell membranes, focusing on the role of membrane\u2010embedded proteins during methylene blue (MB) photooxidation with red light. Membrane extraction was achieved via centrifugation with various sucrose gradients. We then generated GPMVs by electroformation, applying different frequencies and voltages over four cycles, and examined them using phase contrast optical microscopy. For MB photooxidation, GPMVs were dispersed in an aqueous solution with 50\u2009\u03bcM MB and exposed to 665\u2009nm light at 830\u2009\u03bcW. A comparable approach was used for mimetic membranes (giant unilamellar vesicles, GUVs) made of\n                    <jats:italic>Leishmania<\/jats:italic>\n                    membrane lipids. MB photoactivation in GUVs caused a transient increase in membrane area and full permeability. Conversely, GPMVs showed an earlier onset of contrast loss but exhibited less overall contrast reduction and no expansion, indicating that membrane proteins in GPMVs modulate the response to oxidative stress. Real\u2010time monitoring revealed morphological changes in\n                    <jats:italic>L. amazonensis<\/jats:italic>\n                    promastigote cells consistent with apoptosis following photodynamic inactivation.\n                  <\/jats:p>","DOI":"10.1111\/php.70000","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T03:05:49Z","timestamp":1749524749000},"page":"480-488","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Developing giant plasma membrane vesicles from\n                    <i>Leishmania<\/i>\n                    cells to investigate the role of membrane proteins in photodynamic inactivation"],"prefix":"10.1111","volume":"102","author":[{"given":"Maressa D. F.","family":"de Souza","sequence":"first","affiliation":[{"name":"Centro de Lasers e Aplica\u00e7\u00f5es Instituto de Pesquisas Energ\u00e9ticas e Nucleares (IPEN\u2010CNEN)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Ciancaglini","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica, Faculdade de Filosofia, Ci\u00eancias e Letras de Ribeir\u00e3o Preto Universidade de S\u00e3o Paulo  Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosangela","family":"Itri","sequence":"additional","affiliation":[{"name":"Departmento de F\u00edsica Aplicada, Instituto de F\u00edsica Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4203-1134","authenticated-orcid":false,"given":"Martha S.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Centro de Lasers e Aplica\u00e7\u00f5es Instituto de Pesquisas Energ\u00e9ticas e Nucleares (IPEN\u2010CNEN)  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/S12551-023-01066-5"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12551-013-0128-9"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2013.11.4457"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2019.106263"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/ACSOMEGA.9B03244"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2021.112236"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/S10103-024-04174-0"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/MICROORGANISMS11122845"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.MOLBIOPARA.2009.12.004"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/9783527694082.ch15"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/DC9868100303"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008469200"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/S00232-006-0005-6"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.JCIS.2022.05.145"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.EXPPARA.2020.107964"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2011.577540"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20190044"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3sm52740a"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/10715762.2023.2201391"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pdpdt.2018.05.005"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1051\/PARASITE\/2019071"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/PHP.13685"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/S13628-017-0041-X"}],"container-title":["Photochemistry and Photobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/php.70000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/php.70000","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/php.70000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T16:43:36Z","timestamp":1780505016000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/php.70000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,10]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/php.70000"],"URL":"https:\/\/doi.org\/10.1111\/php.70000","archive":["Portico"],"relation":{},"ISSN":["0031-8655","1751-1097"],"issn-type":[{"value":"0031-8655","type":"print"},{"value":"1751-1097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,10]]},"assertion":[{"value":"2024-11-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}