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When internalized by cells, 6\u2010TGua is metabolized through the purine salvage pathway and readily incorporated into DNA. Patients treated with these thiopurines are more prone to develop squamous cell carcinoma of the skin. The absorbance spectrum of 6\u2010TGua, in contrast to guanine (Gua) or any other canonical base, has a maximum absorbance at 342\u2009nm. Therefore, 6\u2010TGua undergoes photoexcitation upon exposure to UVA radiation, with maximum absorption at 340\u2009nm. In this study, the used approach unequivocally demonstrates the generation and quenching of\n                    <jats:sup>1<\/jats:sup>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    by 6\u2010TGua via the direct spectroscopic detection of\n                    <jats:sup>1<\/jats:sup>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    monomol light emission at 1270\u2009nm. Chemiluminescence\u2010based methods were employed for the determination of the\n                    <jats:sup>1<\/jats:sup>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    generation quantum yield (\u03a6\n                    <jats:sup>1<\/jats:sup>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    ) and the total\n                    <jats:sup>1<\/jats:sup>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    quenching rate constant (k\n                    <jats:sub>\n                      <jats:italic>t<\/jats:italic>\n                    <\/jats:sub>\n                    ). For the\n                    <jats:sup>1<\/jats:sup>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    quantum yield, we found values of 0.22\u2009\u00b1\u20090.03 for 6\u2010TGua and 0.12\u2009\u00b1\u20090.03 for 2\u2032\u2010deoxy\u20106\u2010thioguanosine (6\u2010TdGuo). These compounds presented k\n                    <jats:sub>\n                      <jats:italic>t<\/jats:italic>\n                    <\/jats:sub>\n                    values of 1.5\u2009\u00d7\u200910\n                    <jats:sup>7<\/jats:sup>\n                    \u2009L\u2009mol\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u2009s\n                    <jats:sup>\u22121<\/jats:sup>\n                    and 1.1\u2009\u00d7\u200910\n                    <jats:sup>7<\/jats:sup>\n                    \u2009L\u2009mol\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u2009s\n                    <jats:sup>\u22121<\/jats:sup>\n                    , respectively. Through the comparison of these values with the ones obtained for 2\u2032\u2010deoxyguanosine (dGuo) and its oxidation product 8\u2010oxo\u20107,8\u2010dihydro\u20102\u2032\u2010deoxyguanosine (8\u2010oxodGuo), we aim to provide new insights into the 6\u2010TGua\u2010containing DNA (DNA\u20106\u2010TGua) reactivity towards\n                    <jats:sup>1<\/jats:sup>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    in a biological context.\n                  <\/jats:p>","DOI":"10.1111\/php.70044","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T09:35:19Z","timestamp":1764322519000},"page":"605-614","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Direct evidence of singlet molecular oxygen [O\n                    <sub>2<\/sub>\n                    (\n                    <scp>\n                      <sup>1<\/sup>\n                      \u0394g\n                    <\/scp>\n                    )] production from\n                    <scp>UVA<\/scp>\n                    excited 6\u2010thioguanine"],"prefix":"10.1111","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0395-5481","authenticated-orcid":false,"given":"Andr\u00e9 L.","family":"Lopes","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Institute of Chemistry Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4458-4354","authenticated-orcid":false,"given":"Fernanda M.","family":"Prado","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Institute of Chemistry Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0159-0191","authenticated-orcid":false,"given":"Helena C.","family":"Junqueira","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Institute of Chemistry Universidade de S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5438-1174","authenticated-orcid":false,"given":"Marisa H. 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