{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T12:06:37Z","timestamp":1789733197262,"version":"4.0.1"},"reference-count":56,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22373010"],"award-info":[{"award-number":["22373010"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22403008"],"award-info":[{"award-number":["22403008"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21973005"],"award-info":[{"award-number":["21973005"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005089","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["2244074"],"award-info":[{"award-number":["2244074"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2025A1515012183"],"award-info":[{"award-number":["2025A1515012183"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Photochem &amp;amp; Photobiology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Firefly bioluminescence (BL) is widely utilized in bioimaging due to its high quantum yield; however, its performance in long\u2010term monitoring is limited by rapid signal decay resulting from product inhibition by oxyluciferin (\n                    <jats:bold>oLu<\/jats:bold>\n                    ). In nature, fireflies employ the luciferin\u2010regenerating enzyme (LRE) to catalyze\n                    <jats:bold>oLu<\/jats:bold>\n                    hydrolysis, thereby removing inhibition and initiating substrate regeneration for subsequent emission. While previous studies have established the three\u2010step regeneration pathway\u2014LRE\u2010catalyzed\n                    <jats:bold>oLu<\/jats:bold>\n                    hydrolysis, CHBT condensation with L\u2010Cys, and L\/D\u2010luciferin isomerization\u2014the initiating step lacks molecular\u2010level clarity. Specifically, how the active site environment of LRE facilitates this reaction remains unknown, which hinders a comprehensive understanding of the recycling process and the rational optimization of sustained bioluminescent systems. To address this, we investigate its microscopic mechanism using molecular dynamics (MD) simulations and quantum mechanics\/molecular mechanics (QM\/MM) calculations. The results reveal that LRE provides a critical residue, Asp111, which acts as a general base to facilitate nucleophilic addition. The subsequent ring opening follows a stepwise mechanism, characterized by sequential C4\u2010N3 bond cleavage and proton transfer\u2010coupled C2\u2010S1 bond breaking as the rate\u2010determining step. Overall, this work elucidates the molecular role of LRE in substrate regeneration and provides a theoretical foundation for understanding how fireflies restart their bioluminescent cycles.\n                  <\/jats:p>","DOI":"10.1111\/php.70152","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:50:39Z","timestamp":1789732239000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Theoretical study on the catalytic mechanism of oxyluciferin hydrolysis by luciferin\u2010regenerating enzyme in firefly bioluminescence substrate regeneration"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9773-5618","authenticated-orcid":false,"given":"Jinyu","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry Beijing Normal University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7161-1253","authenticated-orcid":false,"given":"Deping","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Arts and Sciences, Center for Advanced Materials Research Beijing Normal University  Zhuhai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8761-5339","authenticated-orcid":false,"given":"Ya\u2010Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Theoretical and Computational Photochemistry, Ministry of Education, College of Chemistry Beijing Normal University  Beijing China"},{"name":"Department of Chemistry, Faculty of Arts and Sciences, Center for Advanced Materials Research Beijing Normal University  Zhuhai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","first-page":"5610","DOI":"10.1021\/acs.analchem.6b01160","article-title":"Real\u2010time bioluminescence imaging of nitroreductase in mouse model","volume":"88","author":"Feng P","year":"2016","journal-title":"Anal Chem"},{"key":"e_1_2_8_3_1","doi-asserted-by":"crossref","first-page":"7410","DOI":"10.1021\/ja4013535","article-title":"Mechanism\u2010based design of a photoactivatable firefly luciferase","volume":"135","author":"Zhao J","year":"2013","journal-title":"J Am Chem Soc"},{"key":"e_1_2_8_4_1","doi-asserted-by":"crossref","first-page":"1774","DOI":"10.1039\/C7CC09720D","article-title":"An allylated firefly luciferin analogue with luciferase specific response in living cells","volume":"54","author":"Ikeda Y","year":"2018","journal-title":"Chem Commun"},{"key":"e_1_2_8_5_1","doi-asserted-by":"crossref","DOI":"10.3390\/bios12060400","article-title":"Bioluminescence color\u2010tuning firefly luciferases: engineering and prospects for real\u2010time intracellular pH imaging and heavy metal biosensing","volume":"12","author":"Viviani VR","year":"2022","journal-title":"Biosensors (Basel)"},{"key":"e_1_2_8_6_1","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nphoton.2007.251","article-title":"Firefly bioluminescence quantum yield and colour change by pH\u2010sensitive green emission","volume":"2","author":"Ando Y","year":"2007","journal-title":"Nat Photonics"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"3671","DOI":"10.1002\/chem.201503944","article-title":"Design and synthesis of an alkynyl luciferin analogue for bioluminescence imaging","volume":"22","author":"Steinhardt RC","year":"2016","journal-title":"Chem Eur J"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jphotochem.2022.114270","article-title":"Quantum yield of near\u2010infrared bioluminescence with firefly luciferin analog: AkaLumine","volume":"434","author":"Ono R","year":"2023","journal-title":"J Photochem Photobiol A"},{"key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"13059","DOI":"10.1002\/anie.201405955","article-title":"A dual\u2010color far\u2010red to near\u2010infrared firefly luciferin analogue designed for multiparametric bioluminescence imaging","volume":"53","author":"Jathoul AP","year":"2014","journal-title":"Angew Chem Int Ed"},{"key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1021\/acs.biochem.3c00708","article-title":"Discovery of red\u2010shifting mutations in Firefly luciferase using high\u2010throughput biochemistry","volume":"63","author":"Colee CM","year":"2024","journal-title":"Biochemistry"},{"key":"e_1_2_8_11_1","doi-asserted-by":"crossref","first-page":"2061","DOI":"10.1039\/c9pp00174c","article-title":"A highly efficient, thermostable and cadmium selective firefly luciferase suitable for ratiometric metal and pH biosensing and for sensitive ATP assays","volume":"18","author":"Pelentir GF","year":"2020","journal-title":"Photochem Photobiol Sci"},{"key":"e_1_2_8_12_1","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1021\/bi00702a015","article-title":"Kinetics of the firefly luciferase catalyzed reactions","volume":"13","author":"DeLuca M","year":"1974","journal-title":"Biochemistry"},{"key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/0003-9861(75)90205-2","article-title":"The production of oxyluciferin during the firefly luciferase light reaction","volume":"169","author":"Gates BJ","year":"1975","journal-title":"Arch Biochem Biophys"},{"key":"e_1_2_8_14_1","doi-asserted-by":"crossref","first-page":"1085","DOI":"10.1039\/b809935a","article-title":"Kinetics of inhibition of firefly luciferase by oxyluciferin and dehydroluciferyl\u2010adenylate","volume":"7","author":"Ribeiro C","year":"2008","journal-title":"Photochem Photobiol Sci"},{"key":"e_1_2_8_15_1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1093\/beheco\/14.1.135","article-title":"Female preference for male courtship flashes in Photinus ignitus fireflies","volume":"14","author":"Lewis CK","year":"2003","journal-title":"Behav Ecol"},{"key":"e_1_2_8_16_1","doi-asserted-by":"crossref","first-page":"36508","DOI":"10.1074\/jbc.M105528200","article-title":"Oxyluciferin, a luminescence product of firefly luciferase, is enzymatically regenerated into luciferin","volume":"276","author":"Gomi K","year":"2001","journal-title":"J Biol Chem"},{"key":"e_1_2_8_17_1","doi-asserted-by":"crossref","first-page":"2771","DOI":"10.1016\/S0040-4039(01)91738-1","article-title":"Firefly bioluminescence III. Conversion of oxyluciferin to luciferin in firefly","volume":"15","author":"Okada K","year":"1974","journal-title":"Tetrahedron Lett"},{"key":"e_1_2_8_18_1","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1039\/c39760000153","article-title":"Biosynthesis of luciferin in Pyrophorus pellucens","volume":"5","author":"McCapra F","year":"1976","journal-title":"J Chem Soc Chem Commun"},{"key":"e_1_2_8_19_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.bios.2022.114860","article-title":"Realization of firefly bioluminescence cycle in\u00a0vitro and in cells","volume":"220","author":"Zheng Z","year":"2023","journal-title":"Biosens Bioelectron"},{"key":"e_1_2_8_20_1","doi-asserted-by":"crossref","first-page":"5283","DOI":"10.1016\/j.febslet.2006.08.073","article-title":"Stereoisomeric bio\u2010inversion key to biosynthesis of firefly D\u2010luciferin","volume":"580","author":"Niwa K","year":"2006","journal-title":"FEBS Lett"},{"key":"e_1_2_8_21_1","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/j.bbrc.2005.03.202","article-title":"Firefly luciferase exhibits bimodal action depending on the luciferin chirality","volume":"331","author":"Nakamura M","year":"2005","journal-title":"Biochem Biophys Res Commun"},{"key":"e_1_2_8_22_1","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1021\/cb900156h","article-title":"Conformational dynamics in the acyl\u2010CoA synthetases, adenylation domains of non\u2010ribosomal peptide synthetases, and firefly luciferase","volume":"4","author":"Gulick AM","year":"2009","journal-title":"ACS Chem Biol"},{"key":"e_1_2_8_23_1","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1002\/cphc.201900306","article-title":"Luciferin regeneration in Firefly bioluminescence via proton\u2010transfer\u2010facilitated hydrolysis, condensation and chiral inversion","volume":"20","author":"Cheng YY","year":"2019","journal-title":"ChemPhysChem"},{"key":"e_1_2_8_24_1","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.enzmictec.2010.05.008","article-title":"RACE\u2010based amplification of cDNA and expression of a luciferin\u2010regenerating enzyme (LRE): an attempt towards persistent bioluminescent signal","volume":"47","author":"Emamzadeh R","year":"2010","journal-title":"Enzym Microb Technol"},{"key":"e_1_2_8_25_1","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/j.ab.2009.09.014","article-title":"Applications of luciferin biosynthesis: bioluminescence assays for L\u2010cysteine and luciferase","volume":"396","author":"Niwa K","year":"2010","journal-title":"Anal Biochem"},{"key":"e_1_2_8_26_1","doi-asserted-by":"crossref","first-page":"2112","DOI":"10.1016\/j.bbagen.2017.04.010","article-title":"Biosynthesis\u2010inspired deracemizative production of d\u2010luciferin by combining luciferase and thioesterase","volume":"1861","author":"Maeda J","year":"2017","journal-title":"Biochim Biophys Acta, Gen Subj"},{"key":"e_1_2_8_27_1","doi-asserted-by":"crossref","first-page":"3479","DOI":"10.1039\/C7OB00603A","article-title":"Mechanistic model for the firefly luciferin regeneration in biomimetic conditions: a model for the in\u00a0vivo process?","volume":"15","author":"Salatino CT","year":"2017","journal-title":"Org Biomol Chem"},{"key":"e_1_2_8_28_1","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1039\/C3OB41241E","article-title":"A biocompatible, highly efficient click reaction and its applications","volume":"12","author":"Yuan Y","year":"2014","journal-title":"Org Biomol Chem"},{"key":"e_1_2_8_29_1","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-020-72900-z","article-title":"Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies","volume":"10","author":"Zhang R","year":"2020","journal-title":"Sci Rep"},{"key":"e_1_2_8_30_1","doi-asserted-by":"crossref","DOI":"10.1038\/srep14017","article-title":"An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography","volume":"5","author":"Yamashita K","year":"2015","journal-title":"Sci Rep"},{"key":"e_1_2_8_31_1","doi-asserted-by":"crossref","first-page":"W368","DOI":"10.1093\/nar\/gki464","article-title":"H++: a server for estimating pKas and adding missing hydrogens to macromolecules","volume":"33","author":"Gordon JC","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"e_1_2_8_32_1","doi-asserted-by":"crossref","first-page":"3264","DOI":"10.1073\/pnas.84.10.3264","article-title":"Firefly luciferase is targeted to peroxisomes in mammalian cells","volume":"84","author":"Keller GA","year":"1987","journal-title":"Proc Natl Acad Sci USA"},{"issue":"1","key":"e_1_2_8_33_1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1038\/71375","article-title":"Peroxisomes in human fibroblasts have a basic pH","volume":"2","author":"Dansen TB","year":"2000","journal-title":"Nat Cell Biol"},{"key":"e_1_2_8_34_1","doi-asserted-by":"crossref","first-page":"3891","DOI":"10.1021\/acs.jcim.1c00203","article-title":"AutoDock Vina 1.2.0: new docking methods, expanded force field, and python bindings","volume":"61","author":"Eberhardt J","year":"2021","journal-title":"J Chem Inf Model"},{"key":"e_1_2_8_35_1","doi-asserted-by":"crossref","first-page":"2141","DOI":"10.1016\/j.csbj.2024.05.024","article-title":"Addressing docking pose selection with structure\u2010based deep learning: recent advances, challenges and opportunities","volume":"23","author":"Vittorio S","year":"2024","journal-title":"Comput Struct Biotechnol J"},{"key":"e_1_2_8_36_1","doi-asserted-by":"crossref","DOI":"10.1002\/adsc.70232","article-title":"Engineering of an ancestral McbA with enhanced domain mobility extends biocatalytic amide synthesis scope","volume":"368","author":"S\u00f6derberg E","year":"2026","journal-title":"Adv Synth Catal"},{"key":"e_1_2_8_37_1","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1016\/j.csbj.2024.12.004","article-title":"PUR\u2010GEN: a web server for automated generation of polyurethane fragment libraries","volume":"27","author":"Szleper K","year":"2024","journal-title":"Comput Struct Biotechnol J"},{"key":"e_1_2_8_38_1","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1021\/acs.jctc.9b00591","article-title":"ff19SB: amino\u2010acid\u2010specific protein backbone parameters trained against quantum mechanics energy surfaces in solution","volume":"16","author":"Tian C","year":"2019","journal-title":"J Chem Theory Comput"},{"key":"e_1_2_8_39_1","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1002\/jcc.20035","article-title":"Development and testing of a general amber force field","volume":"25","author":"Wang JM","year":"2004","journal-title":"J Comput Chem"},{"key":"e_1_2_8_40_1","volume-title":"Gaussian 09, Revision D.01","author":"Frisch MJ","year":"2009"},{"key":"e_1_2_8_41_1","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1002\/jcc.540161106","article-title":"Application of the multimolecule and multiconformational RESP methodology to biopolymers: charge derivation for DNA, RNA, and proteins","volume":"16","author":"Cieplak P","year":"1995","journal-title":"J Comput Chem"},{"key":"e_1_2_8_42_1","doi-asserted-by":"crossref","first-page":"926","DOI":"10.1063\/1.445869","article-title":"Comparison of simple potential functions for simulating liquid water","volume":"79","author":"Jorgensen WL","year":"1983","journal-title":"J Chem Phys"},{"key":"e_1_2_8_43_1","doi-asserted-by":"crossref","first-page":"2090","DOI":"10.1063\/1.1332996","article-title":"Langevin stabilization of molecular dynamics","volume":"114","author":"Izaguirre JA","year":"2001","journal-title":"J Chem Phys"},{"key":"e_1_2_8_44_1","doi-asserted-by":"crossref","first-page":"3684","DOI":"10.1063\/1.448118","article-title":"Molecular dynamics with coupling to an external bath","volume":"81","author":"Berendsen HJC","year":"1984","journal-title":"J Chem Phys"},{"key":"e_1_2_8_45_1","doi-asserted-by":"crossref","first-page":"10089","DOI":"10.1063\/1.464397","article-title":"Particle mesh Ewald: an N\u00b7log(N) method for Ewald sums in large systems","volume":"98","author":"Darden T","year":"1993","journal-title":"J Chem Phys"},{"key":"e_1_2_8_46_1","first-page":"327","article-title":"Numerical integration of the cartesian equations of motion of a system with constraints: molecular dynamics of n\u2010alkanes","volume":"23","author":"Ryckaert JP","year":"1977","journal-title":"J Chem Phys"},{"key":"e_1_2_8_47_1","volume-title":"Amber 2021","author":"MacKerell AD","year":"2021"},{"key":"e_1_2_8_48_1","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1002\/jcc.21372","article-title":"Fast and accurate predictions of binding free energies using MM\u2010PBSA and MM\u2010GBSA","volume":"31","author":"Rastelli G","year":"2010","journal-title":"J Comput Chem"},{"key":"e_1_2_8_49_1","doi-asserted-by":"crossref","first-page":"6493","DOI":"10.1021\/bi300934s","article-title":"Crystal structure of firefly luciferase in a second catalytic conformation supports a domain alternation mechanism","volume":"51","author":"Sundlov JA","year":"2012","journal-title":"Biochemistry"},{"key":"e_1_2_8_50_1","doi-asserted-by":"crossref","first-page":"10580","DOI":"10.1021\/jp9536514","article-title":"Hybrid models for combined quantum mechanical and molecular mechanical approaches","volume":"100","author":"Bakowies D","year":"1996","journal-title":"J Phys Chem"},{"key":"e_1_2_8_51_1","doi-asserted-by":"crossref","first-page":"7212","DOI":"10.1039\/C9SC01464K","article-title":"Conformational diversity induces nanosecond\u2010timescale chemical disorder in the HIV\u20101 protease reaction pathway","volume":"10","author":"Calixto AR","year":"2019","journal-title":"Chem Sci"},{"key":"e_1_2_8_52_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/S0166-1280(03)00285-9","article-title":"QUASI: a general purpose implementation of the QM\/MM approach and its application to problems in catalysis","volume":"632","author":"Sherwood P","year":"2003","journal-title":"J Mol Struct THEOCHEM"},{"key":"e_1_2_8_53_1","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/wcms.1163","article-title":"ChemShell\u2014a modular software package for QM\/MM simulations","volume":"4","author":"Metz S","year":"2013","journal-title":"WIREs Comput Mol Sci"},{"key":"e_1_2_8_54_1","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/wcms.1606","article-title":"Software update: the ORCA program system\u2014version 5.0","volume":"12","author":"Neese F","year":"2022","journal-title":"WIREs Comput Mol Sci"},{"key":"e_1_2_8_55_1","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/S0263-7855(96)00043-4","article-title":"DL_POLY_2.0: a general\u2010purpose parallel molecular dynamics simulation package","volume":"14","author":"Smith W","year":"1996","journal-title":"J Mol Graph"},{"key":"e_1_2_8_56_1","doi-asserted-by":"crossref","DOI":"10.1002\/wcms.1494","article-title":"TeraChem: a graphical processing unit\u2010accelerated electronic structure package for large\u2010scale ab\u00a0initio molecular dynamics","volume":"11","author":"Seritan S","year":"2021","journal-title":"WIREs Comput Mol Sci"},{"key":"e_1_2_8_57_1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/1011-1344(93)06970-E","article-title":"Investigation of the interaction between firefly luciferase and oxyluciferin or its analogues by steady state and subnanosecond time\u2010resolved fluorescence","volume":"22","author":"Gandelman OA","year":"1994","journal-title":"J Photochem Photobiol B"}],"container-title":["Photochemistry and Photobiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/php.70152","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/php.70152","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/php.70152","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T11:50:47Z","timestamp":1789732247000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/php.70152"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":56,"alternative-id":["10.1111\/php.70152"],"URL":"https:\/\/doi.org\/10.1111\/php.70152","archive":["Portico"],"relation":{},"ISSN":["0031-8655","1751-1097"],"issn-type":[{"value":"0031-8655","type":"print"},{"value":"1751-1097","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,18]]},"assertion":[{"value":"2026-06-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"php.70152"}}