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John Stanton, Jr. CTCL Research Fund"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Extracorporeal photopheresis (<jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content>) alone or in combination therapy is effective for treatment of leukemic cutaneous T\u2010cell lymphoma (<jats:styled-content style=\"fixed-case\">L\u2010CTCL<\/jats:styled-content>), but its mechanism(s) of action remain unclear. This study was designed to investigate the effect of<jats:styled-content style=\"fixed-case\">ECP<\/jats:styled-content>on regulatory T cells and CD8<jats:sup>+<\/jats:sup>T cells in<jats:styled-content style=\"fixed-case\">L\u2010CTCL<\/jats:styled-content>patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Experimental Design<\/jats:title><jats:p>Peripheral blood from 18\u00a0L\u2010CTCL patients at baseline, Day 2, 1\u00a0month, 3\u00a0month, and 6\u00a0month post\u2010ECP therapy was analyzed by flow cytometry for CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+\/high<\/jats:sup>, CD4<jats:sup>+<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup>CD25<jats:sup>+\/\u2212<\/jats:sup>, CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup>, CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup>CD69<jats:sup>+<\/jats:sup>, and CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup>IFN\u2010\u03b3<jats:sup>+<\/jats:sup>T cells. Clinical responses were assessed and correlated with changes in these T\u2010cell subsets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twelve of 18 patients achieved clinical responses. The average baseline number of CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+\/high<\/jats:sup>T cells of PBMCs in L\u2010CTCL patients was normal (2.2%), but increased at 6\u2010month post\u2010therapy (4.3%,<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01). The average baseline number of CD4<jats:sup>+<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup>T cells out of CD4<jats:sup>+<\/jats:sup>T cells in nine evaluable patients was high (66.8\u00a0\u00b1\u00a013.7%), mostly CD25 negative. The levels of CD4<jats:sup>+<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup>T cells in responders were higher (<jats:italic>n<\/jats:italic>\u00a0=\u00a06, 93.1\u00a0\u00b1\u00a05.7%) than nonresponders (<jats:italic>n<\/jats:italic>\u00a0=\u00a03, 14.2\u00a0\u00b1\u00a016.0%,<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.01), and they declined in parallel with malignant T cells. The numbers of CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup>CD69<jats:sup>+<\/jats:sup>and CD3<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup>IFN\u2010\u03b3<jats:sup>+<\/jats:sup>T cells increased at 3\u2010month post\u2010therapy in five of six patients studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Extracorporeal photopheresis alone or in combination therapy might be effective in<jats:styled-content style=\"fixed-case\">L\u2010CTCL<\/jats:styled-content>patients whose malignant T cells have a CD4<jats:sup>+<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup>CD25<jats:sup>\u2212<\/jats:sup>phenotype.<\/jats:p><\/jats:sec>","DOI":"10.1111\/phpp.12175","type":"journal-article","created":{"date-parts":[[2015,3,13]],"date-time":"2015-03-13T12:26:25Z","timestamp":1426249585000},"page":"184-194","source":"Crossref","is-referenced-by-count":16,"title":["The effect of extracorporeal photopheresis alone or in combination therapy on circulating<scp>CD<\/scp>4<sup>+<\/sup>Foxp3<sup>+<\/sup><scp>CD<\/scp>25<sup>\u2212<\/sup>T cells in patients with leukemic cutaneous T\u2010cell lymphoma"],"prefix":"10.1111","volume":"31","author":[{"given":"Lisa H.","family":"Shiue","sequence":"first","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center Houston TX USA"},{"name":"Graduate Program in Immunology Graduate School of Biomedical Sciences University of Texas Health Science Center at Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Couturier","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine University of Texas Health Science Center at Houston Houston TX USA"},{"name":"Graduate Program in Virology Graduate School of Biomedical Sciences University of Texas Health Science Center at Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothy E.","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine University of Texas Health Science Center at Houston Houston TX USA"},{"name":"Graduate Program in Virology Graduate School of Biomedical Sciences University of Texas Health Science Center at Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caimiao","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Biostatistics The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center Houston TX USA"},{"name":"Graduate Program in Immunology Graduate School of Biomedical Sciences University of Texas Health Science Center at Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeleine","family":"Duvic","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center Houston TX USA"},{"name":"Graduate Program in Immunology Graduate School of Biomedical Sciences University of Texas Health Science Center at Houston Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-09-3502"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.3.624"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2009.03771.x"},{"key":"e_1_2_6_5_1","unstructured":"TalpurRDS SeyferS LiuP DuvicM.Long\u2010term outcomes of 1263 patients with mycosis fungoides and Sezary syndrome from 1982\u20102009. 1st World Congress of Cutaneous Lymphoma. 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