{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T22:25:23Z","timestamp":1789597523985,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T00:00:00Z","timestamp":1487808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"IFC"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2017,5]]},"abstract":"Summary<\/jats:title>Background<\/jats:title>Polypodium leucotomos<\/jats:italic> (PL<\/jats:styled-content>) exerts potent antioxidant, photo\u2010protective, and immune\u2010modulatory activities. A reconstructed human epidermis (RHE<\/jats:styled-content>) (Episkin) is a suitable model for the evaluation of acute UV<\/jats:styled-content>\u2010induced cell damage. No data regarding the photo\u2010protective action of PL<\/jats:styled-content> in this model are available.<\/jats:p><\/jats:sec>Purpose<\/jats:title>We evaluated the effects of PL<\/jats:styled-content> on the prevention of UVB<\/jats:styled-content>\u2010induced cell damage assessing sunburn cells, CPD<\/jats:styled-content> formation, p53, Ki\u201067, p21 expression, and epidermal growth factor (EGF<\/jats:styled-content>) production.<\/jats:p><\/jats:sec>Materials & Methods<\/jats:title>RHE<\/jats:styled-content> was incubated in standard conditions. PL<\/jats:styled-content> was topically applied at the concentration of 2 mg\/cm2<\/jats:sup>, immediately before UVB<\/jats:styled-content> exposition. UVB<\/jats:styled-content> exposition (300 mJ\/cm2<\/jats:sup>) was performed using a dedicated UVB<\/jats:styled-content> lamp. Irradiated samples without PL<\/jats:styled-content> and non\u2010irradiated samples were used as positive and negative controls. Expression of p53, p21, and Ki\u201067 was evaluated with immune\u2010histochemical methods. CPD<\/jats:styled-content> were measured using a monoclonal antibody.<\/jats:p><\/jats:sec>Results<\/jats:title>PL<\/jats:styled-content> significantly reduced sunburned cells (\u221280%) in comparison with positive control. PL<\/jats:styled-content> significantly prevented the increase in EGF<\/jats:styled-content> production at tested times. PL<\/jats:styled-content> significantly reduced the p53 (\u221280%), p21 (\u221284%), and Ki\u201067 (\u221248%) positive cells. Finally, PL<\/jats:styled-content> prevented the formation of CPD<\/jats:styled-content> (0% vs. 20% positive cells).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>In this model, PL<\/jats:styled-content> has shown to prevent UVB<\/jats:styled-content> cell damage, the upregulation of proliferating proteins, and fully blocking the formation of CPD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/phpp.12297","type":"journal-article","created":{"date-parts":[[2017,1,31]],"date-time":"2017-01-31T13:59:06Z","timestamp":1485871146000},"page":"156-163","source":"Crossref","is-referenced-by-count":19,"title":["Protective effects of Polypodium leucotomos<\/i> extract against UVB<\/scp>\u2010induced damage in a model of reconstructed human epidermis"],"prefix":"10.1111","volume":"33","author":[{"given":"Paola","family":"Torricelli","sequence":"first","affiliation":[{"name":"Laboratory of Biocompatibility Innovative Technologies and Advanced Therapies (BITTA) Rizzoli Orthopaedic 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