{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:08:14Z","timestamp":1791558494299,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,7,23]],"date-time":"2018-07-23T00:00:00Z","timestamp":1532304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006479","name":"Cordis","doi-asserted-by":"publisher","award":["Focused giving program"],"award-info":[{"award-number":["Focused giving program"]}],"id":[{"id":"10.13039\/100006479","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["NIH R01AI106874"],"award-info":[{"award-number":["NIH R01AI106874"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:sec><jats:title>Background\/Purpose<\/jats:title><jats:p>Skin commensal bacteria have been described to help orchestrate skin homeostasis, signaling through innate immunity pathways. This study for the first time aimed at studying the relationship between skin commensals and melanocytes after <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content> exposure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An in vitro <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content> radiation model with normal human epidermal melanocytes (<jats:styled-content style=\"fixed-case\">NHM<\/jats:styled-content>s) and skin commensal bacteria supernatant from <jats:italic>Staphylococcus epidermidis<\/jats:italic> and <jats:italic>Propionibacterium acnes<\/jats:italic> was established. Melanocytes <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage, cyclobutane pyrimidine dimers (<jats:styled-content style=\"fixed-case\">CPD<\/jats:styled-content>), and cellular proliferation marker Ki\u201067 were measured by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> and immunofluorescence staining. Cell apoptosis was assessed by flow cytometry and <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> array and <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Normal human epidermal melanocytes are able to survive and proliferate while bearing <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> damage after <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content> radiation. Skin commensal bacteria <jats:italic>S.\u00a0epidermidis<\/jats:italic> and its by\u2010product <jats:styled-content style=\"fixed-case\">LTA<\/jats:styled-content> promote melanocytes survival by inducing upregulation of <jats:styled-content style=\"fixed-case\">TRAF<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">CASP<\/jats:styled-content>14, <jats:styled-content style=\"fixed-case\">CASP<\/jats:styled-content>5, and <jats:styled-content style=\"fixed-case\">TP<\/jats:styled-content>73. On the other hand, <jats:italic>P.\u00a0acnes<\/jats:italic> can inhibit <jats:styled-content style=\"fixed-case\">UVB<\/jats:styled-content>\u2010irradiated melanocytes survival by increasing apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our studies show different aspects of commensal activity on melanocytes during irradiation. The possible balance achieved by the different skin commensal can influence <jats:styled-content style=\"fixed-case\">NHM<\/jats:styled-content> potential to become cancer cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/phpp.12411","type":"journal-article","created":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T08:22:57Z","timestamp":1530778977000},"page":"405-414","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Skin commensal bacteria <i>Staphylococcus epidermidis<\/i> promote survival of melanocytes bearing <scp>UVB<\/scp>\u2010induced <scp>DNA<\/scp> damage, while bacteria <i>Propionibacterium acnes<\/i> inhibit survival of melanocytes by increasing apoptosis"],"prefix":"10.1111","volume":"34","author":[{"given":"Zhenping","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Dermatology University of California  San Diego California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae\u2010Eun","family":"Choi","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California  San Diego California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia\u2010Chi","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California  San Diego California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5575-9968","authenticated-orcid":false,"given":"Anna","family":"Di Nardo","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of California  San Diego California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,23]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(01)00199-3"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2009.03.010"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.2000.4322"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1383-5742(02)00009-1"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05529"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-014-0452-6"},{"issue":"1205","key":"e_1_2_5_8_1","article-title":"Skin microbiome promotes mast cell maturation by triggering stem cell factor production in keratinocytes","volume":"139","author":"Wang Z","year":"2017","journal-title":"J Allergy Clin Immunol"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2062"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aah4609"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2861"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.990170108"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1093\/clinchem\/23.2.264","article-title":"Free erythrocyte porphyrins in the detection of undue absorption of Pb and of Fe deficiency","volume":"23","author":"Piomelli S","year":"1977","journal-title":"Clin Chem"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.4.1117"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5383.1680"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1172308"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2005.06530.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/b512837d"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/exd.12482"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2768"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature00766"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.423"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1983.tb07077.x"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1984.tb07324.x"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2537"},{"key":"e_1_2_5_27_1","first-page":"591","article-title":"Vital signs: melanoma incidence and mortality trends and projections \u2010 United States, 1982\u20102030","volume":"64","author":"Guy GP","year":"2015","journal-title":"MMWR Morb Mortal Wkly Rep"}],"container-title":["Photodermatology, Photoimmunology &amp; 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