{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T14:10:34Z","timestamp":1768918234615,"version":"3.49.0"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,4,16]],"date-time":"2020-04-16T00:00:00Z","timestamp":1586995200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007163","name":"Therakos, Inc","doi-asserted-by":"crossref","award":["IIS-TKS-2012-007\/Ni"],"award-info":[{"award-number":["IIS-TKS-2012-007\/Ni"]}],"id":[{"id":"10.13039\/100007163","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Institutes of Health -\u00a0Cancer Center Support Grant","award":["P30 CA016672"],"award-info":[{"award-number":["P30 CA016672"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background\/Purpose<\/jats:title><jats:p>We previously reported that myeloid dendritic cells (mDC) were increased in patients with leukemic cutaneous T\u2010cell lymphoma (L\u2010CTCL) following extracorporeal photopheresis (ECP) using the Therakos UVAR XTS<jats:sup>\u2122<\/jats:sup> system. We now assessed monocyte\u2010derived mDCs (Mo\u2010DCs) in L\u2010CTCL patients treated with the CELLEX<jats:sup>TM<\/jats:sup> photopheresis system. CD209, a transmembrane receptor, was used to define Mo\u2010DCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peripheral blood samples from baseline pre\u2010ECP and at Day 2, 1\u00a0month, 3\u00a0months, and 6\u00a0months post\u2010ECP were analyzed by flow cytometry for Lin<jats:sup>\u2212<\/jats:sup>HLA\u2010DR<jats:sup>+<\/jats:sup>CD123<jats:sup>+<\/jats:sup> plasmacytoid dendritic cells (pDCs), Lin<jats:sup>\u2212<\/jats:sup>HLA\u2010DR<jats:sup>+<\/jats:sup>CD11c<jats:sup>+<\/jats:sup> mDCs, and CD209<jats:sup>+<\/jats:sup> mDCs. The expression of CD209 mRNA was assessed by real\u2010time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At baseline, 7 of 19 patients had lower than normal mDCs, and all patients had lower than normal CD209<jats:sup>+<\/jats:sup> mDCs in peripheral blood mononuclear cells (0.005% in patients, n\u00a0=\u00a019, vs 0.50% in healthy donors, n\u00a0=\u00a07, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.0001). The CD209<jats:sup>+<\/jats:sup> mDC numbers only accounted for 3.28% out of total mDCs in patients compared with 66.51% in healthy donors. After treatment, the CD209<jats:sup>+<\/jats:sup> mDC numbers showed increasing trends in patients. The average absolute numbers of CD209<jats:sup>+<\/jats:sup> mDCs went up by 4.8\u2010fold at 3\u00a0months (n\u00a0=\u00a010, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.103) and by 6.4\u2010fold at 6\u00a0months (n\u00a0=\u00a09, <jats:italic>P<\/jats:italic>\u00a0=\u00a0.100). CD209 mRNA expression went up in two patients responsive to therapy, parallel to CD209<jats:sup>+<\/jats:sup> mDC numbers. L\u2010CTCL patients achieved 70% overall clinical response rate (7\/10) following ECP therapy with the CELLEX<jats:sup>TM<\/jats:sup> system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results suggest that the CELLEX<jats:sup>TM<\/jats:sup> photopheresis system is effective for treating L\u2010CTCL patients like the UVAR XTS<jats:sup>\u2122<\/jats:sup> system, and in vivo<jats:italic>\u2010<\/jats:italic>generated Mo\u2010DCs increase following ECP<jats:italic>.<\/jats:italic><\/jats:p><\/jats:sec>","DOI":"10.1111\/phpp.12552","type":"journal-article","created":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T21:43:02Z","timestamp":1584567782000},"page":"290-298","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["CD209<sup>+<\/sup> monocyte\u2010derived myeloid dendritic cells were increased in patients with leukemic cutaneous T\u2010cell lymphoma undergoing extracorporeal photopheresis via the CELLEX<sup>TM<\/sup> system"],"prefix":"10.1111","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0154-446X","authenticated-orcid":false,"given":"Xiao","family":"Ni","sequence":"first","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4568-1367","authenticated-orcid":false,"given":"Michael","family":"Austin","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4247-8147","authenticated-orcid":false,"given":"Timothy","family":"Langridge","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1593-364X","authenticated-orcid":false,"given":"Pierr","family":"Bojaxhi","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8439-3179","authenticated-orcid":false,"given":"Pedram","family":"Bijani","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2700-092X","authenticated-orcid":false,"given":"Xiaohong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8971-4565","authenticated-orcid":false,"given":"Madeleine","family":"Duvic","sequence":"additional","affiliation":[{"name":"Department of Dermatology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,4,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.26900"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1997.00325.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.142"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/phpp.12175"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/039139880002300109"},{"key":"e_1_2_8_7_1","first-page":"322","article-title":"Mechanistic insights into extracorporeal photochemotherapy: 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