{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:28:14Z","timestamp":1790224094173,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T00:00:00Z","timestamp":1714348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Both piperine and a 308\u2010nm excimer laser have significant curative effects on vitiligo. This study mainly explored the molecular mechanism of a 308\u2010nm excimer combined with piperine in regulating melanocyte proliferation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Epidermal melanocytes were cultured in piperine solution, and the cells were irradiated by an XTRAC excimer laser treatment system at 308\u2010nm output monochromatic light. Quantitative real\u2010time polymerase chain reaction (qRT\u2013PCR) and Western blot were for detecting the expression levels of genes or proteins. The 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyl tetrazolium bromide (MTT) and Transwell method was for assessing cell viability and migration capacity. The content of melanin was also detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The combination of the 308\u2010nm excimer laser and piperine enhanced the cell proliferation, migration, and melanin production of melanocytes and upregulated the level of miR\u2010328, and restraint of miR\u2010328 reversed the influence of the 308\u2010nm excimer laser and piperine. Secreted frizzled\u2010related protein 1 (SFRP1) is a direct target gene of miR\u2010328, and miR\u2010328 can inhibit the expression of SFRP1 and elevate the protein level of the Wnt\/\u03b2\u2010catenin signaling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The 308\u2010nm excimer laser combined with piperine may be more efficient than piperine alone in the remedy of vitiligo, and the miR\u2010328\/SFRP1 and Wnt\/\u03b2\u2010catenin pathways are participated in the proliferation, migration, and melanin synthesis of melanocytes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/phpp.12970","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T04:53:44Z","timestamp":1714452824000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Combination of 308\u2010nm excimer laser and piperine promotes melanocyte proliferation, migration, and melanin content 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