{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T23:17:19Z","timestamp":1778887039122,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T00:00:00Z","timestamp":1744070400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013047","name":"Cultivating Plan Program for the Leader in Science and Technology of Yunnan Province","doi-asserted-by":"publisher","award":["ZX2019\u201003\u201002"],"award-info":[{"award-number":["ZX2019\u201003\u201002"]}],"id":[{"id":"10.13039\/501100013047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013047","name":"Cultivating Plan Program for the Leader in Science and Technology of Yunnan Province","doi-asserted-by":"publisher","award":["2017HA010"],"award-info":[{"award-number":["2017HA010"]}],"id":[{"id":"10.13039\/501100013047","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82260625"],"award-info":[{"award-number":["82260625"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270677"],"award-info":[{"award-number":["32270677"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017947","name":"Yunnan Provincial Postdoctoral Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100017947","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Chronic actinic dermatitis (CAD) is a refractory photoallergic skin disease characterized by inflammatory infiltration and UV sensitivity. While the role of microbiome dysbiosis has been established in various inflammatory skin conditions, its contribution to CAD pathogenesis remains unexplored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To characterize the skin microbiome alterations in CAD patients and investigate their potential associations with disease severity and UV sensitivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Skin microbiome samples were collected from 15 CAD patients and 14 matched family controls, covering both photoexposed (PE) and photoprotected (PNE) areas. The microbial community composition was analyzed using 16S rRNA sequencing. Alpha and beta diversity analyses were performed, and correlations between microbial profiles, disease severity, and UV sensitivity were evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CAD patients exhibited significantly reduced microbial diversity compared to controls, particularly in photoexposed areas (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). This reduction in diversity showed a negative correlation with disease severity. Notably, Staphylococcus abundance was significantly increased in CAD lesions and positively correlated with disease severity. Linear Discriminant Analysis identified Staphylococcus as a potential biomarker for CAD. Interestingly, no significant correlations were found between microbial profiles and UV sensitivity, suggesting independent pathogenic mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings reveal significant alterations in the skin microbiome of CAD patients, characterized by reduced diversity and increased Staphylococcus colonization, which correlates with disease severity but not UV sensitivity. These results provide new insights into CAD pathophysiology and suggest potential therapeutic strategies targeting the skin microbiome.<\/jats:p><\/jats:sec>","DOI":"10.1111\/phpp.70022","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T15:39:18Z","timestamp":1744126758000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<scp>16S rRNA<\/scp> Sequencing Reveals Dysbiosis of Skin Microbiome Associated With Disease Severity in Chronic Actinic Dermatitis"],"prefix":"10.1111","volume":"41","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5860-3985","authenticated-orcid":false,"given":"Yunhua","family":"Tu","sequence":"first","affiliation":[{"name":"Department of Dermatology First Affiliated Hospital of Kunming Medical University  Kunming China"},{"name":"Department of Dermatology The Second People's Hospital of Guiyang  Guizhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuangbin","family":"Xu","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, Microbiome Medicine Center Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Department of Bioinformatics School of Basic Medical Sciences, Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Shu","sequence":"additional","affiliation":[{"name":"Department of Dermatology First Affiliated Hospital of Kunming Medical University  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoli","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Dermatology Changzheng Hospital, Naval Medical University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Dermatology First Affiliated Hospital of Kunming Medical University  Kunming China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangchuang","family":"Yu","sequence":"additional","affiliation":[{"name":"Division of Laboratory Medicine, Microbiome Medicine Center Zhujiang Hospital, Southern Medical University  Guangzhou China"},{"name":"Department of Bioinformatics School of Basic Medical Sciences, Southern Medical University  Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"He","sequence":"additional","affiliation":[{"name":"Department of Dermatology First Affiliated Hospital of Kunming Medical University  Kunming 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