{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T05:49:37Z","timestamp":1791524977583,"version":"4.3.4"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009754","name":"Galderma","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009754","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Photoderm Photoimm Photomed"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Daylight photodynamic therapy (DL\u2010PDT) has been combined with transepidermal drug delivery (TED) techniques to enhance photosensitizer penetration. Although studies have suggested improved clinical and histological outcomes with DL\u2010PDT combined with ablative laser, evidence regarding carcinogenesis and dermal remodeling markers remains limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate changes in epidermal carcinogenesis and dermal remodeling markers after standard DL\u2010PDT compared with DL\u2010PDT combined with TED techniques for facial field cancerization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Forty patients were randomized into four groups (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910): standard DL\u2010PDT, DL\u2010PDT with microneedling, DL\u2010PDT with CO\n                      <jats:sub>2<\/jats:sub>\n                      laser, and DL\u2010PDT with microdermabrasion. All underwent two treatment sessions 1\u2009month apart using methyl aminolevulinate cream followed by 2\u2009h of daylight exposure. Skin biopsies were obtained at baseline and 3\u2009months after the second session. Immunohistochemistry evaluated p53, Ki67, Collagens I and III, metalloproteinases (MMP\u20101, MMP\u20103, MMP\u20109), and TIMP\u20101.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A reduction in the vertical extent of Ki67 expression (from two\u2010thirds to one\u2010third of the epidermis) was observed mainly in the microneedling and CO\n                      <jats:sub>2<\/jats:sub>\n                      laser groups, although it was not statistically significant. No significant differences in Ki67 or p53 expression were observed within or between groups. Increased Type III collagen, MMP\u20103, and MMP\u20109 expression and decreased MMP\u20101 and TIMP\u20101 expression were observed, with only TIMP\u20101 reduction being statistically significant (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.041) in the overall analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      A trend toward reduced vertical Ki67 expression suggests potential benefit of DL\u2010PDT combined with microneedling and CO\n                      <jats:sub>2<\/jats:sub>\n                      laser, and favorable changes in dermal remodeling markers were observed overall. These exploratory findings require confirmation in larger randomized studies.\n                    <\/jats:p>\n                    <jats:p>\n                      <jats:bold>Trial Registration:<\/jats:bold>\n                      <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/clinicaltrials.gov\">ClinicalTrials.gov<\/jats:ext-link>\n                      : NCT03963765\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/phpp.70124","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T04:57:59Z","timestamp":1791521879000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Daylight Photodynamic Therapy Alone or Combined With Transepidermal Drug Delivery Techniques for Skin Field Cancerization: An Immunohistochemical Study of Epidermal Carcinogenesis and Dermal Remodeling Markers"],"prefix":"10.1111","volume":"42","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4607-1432","authenticated-orcid":false,"given":"Camila de Oliveira","family":"Bento","sequence":"first","affiliation":[{"name":"Department of Clinical Medicine\u2014Dermatology Fluminense Federal University  Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7480-2530","authenticated-orcid":false,"given":"Luciana","family":"Pantale\u00e3o","sequence":"additional","affiliation":[{"name":"Department of Pathology Fluminense Federal University  Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Porphirio Jos\u00e9 Soares","family":"Filho","sequence":"additional","affiliation":[{"name":"Department of Pathology Fluminense Federal University  Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7784-9905","authenticated-orcid":false,"given":"Ronir Raggio","family":"Luiz","sequence":"additional","affiliation":[{"name":"Department of Statistics Federal University of Rio de Janeiro  Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0884-4190","authenticated-orcid":false,"given":"Ronaldo Altenburg Odebrecht Curi","family":"Gismondi","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine Fluminense Federal University  Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1031-7370","authenticated-orcid":false,"given":"Maria Claudia Almeida","family":"Issa","sequence":"additional","affiliation":[{"name":"Department of Clinical Medicine\u2014Dermatology Fluminense Federal University  Niter\u00f3i Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1002\/1097-0142(195309)6:5<963::AID-CNCR2820060515>3.0.CO;2-Q","article-title":"Field Cancerization in Oral Stratified Squamous Epithelium","volume":"6","author":"Slaughter D. 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