{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T09:08:01Z","timestamp":1789722481335,"version":"4.0.1"},"reference-count":60,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,12,13]],"date-time":"2012-12-13T00:00:00Z","timestamp":1355356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In this study, mice were vaccinated intranasally with recombinant<jats:italic>N. caninum<\/jats:italic>protein disulphide isomerase (NcPDI) emulsified in cholera toxin (CT) or cholera toxin subunit B (CTB) from<jats:italic>Vibrio cholerae<\/jats:italic>. The effects of vaccination were assessed in the murine nonpregnant model and the foetal infection model, respectively. In the nonpregnant mice, previous results were confirmed, in that intranasal vaccination with recNcPDI in CT was highly protective, and low cerebral parasite loads were noted upon real\u2010time PCR analysis. Protection was accompanied by an IgG1\u2010biased anti\u2010NcPDI response upon infection and significantly increased expression of Th2 (IL\u20104\/IL\u201010) and IL\u201017 transcripts in spleen compared with corresponding values in mice treated with CT only. However, vaccination with recNcPDI in CT did not induce significant protection in dams and their offspring. In the dams, increased splenic Th1 (IFN\u2010\u03b3\/IL\u201012) and Th17<jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>expressions was detected. No protection was noted in the groups vaccinated with recNcPDI emulsified in CTB. Thus, vaccination with recNcPDI in CT in nonpregnant mice followed by challenge infection induced a protective Th2\u2010biased immune response, while in the pregnant mouse model, the same vaccine formulation resulted in a Th1\u2010biased inflammatory response and failed to protect dams and their progeny.<\/jats:p>","DOI":"10.1111\/pim.12013","type":"journal-article","created":{"date-parts":[[2012,10,10]],"date-time":"2012-10-10T11:22:22Z","timestamp":1349868142000},"page":"11-20","source":"Crossref","is-referenced-by-count":13,"title":["Differential effects of intranasal vaccination with recombinant Nc<scp>PDI<\/scp>in different mouse models of<i>Neospora caninum<\/i>infection"],"prefix":"10.1111","volume":"35","author":[{"given":"K.","family":"Debache","sequence":"first","affiliation":[{"name":"Institute of Parasitology Vetsuisse Faculty University of Berne Berne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Hemphill","sequence":"additional","affiliation":[{"name":"Institute of Parasitology Vetsuisse Faculty University of Berne Berne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,12,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00031-06"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(01)00284-3"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2004.03.003"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.2460\/javma.1997.210.8.1169","article-title":"Evidence of vertical transmission of Neospora sp Infection in dairy cattle","volume":"210","author":"Anderson ML","year":"1997","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.05.031"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2307\/3283010"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2011.07.010"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01236-07"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004360000306"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0199-7"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-1-4"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182012000066"},{"key":"e_1_2_6_14_1","first-page":"293","volume-title":"20th Proceedings of ACVIM","author":"Choromanski LJ","year":"2002"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/00480169.2011.631171"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182006000485"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.06.006"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182009991259"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2010.01255.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi961865l"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00532-7"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1740-1747.2003"},{"key":"e_1_2_6_23_1","first-page":"919","article-title":"Cholera toxin B subunit modulation of mucosal vaccines for infectious and autoimmune diseases","volume":"11","author":"Langridge W","year":"2010","journal-title":"Curr Opin Investig Drugs"},{"key":"e_1_2_6_24_1","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.2460\/javma.1988.193.10.1259","article-title":"Neonatal Neospora caninum Infection in dogs: isolation of the causative agent and experimental transmission","volume":"193","author":"Dubey JP","year":"1988","journal-title":"J Am Vet Med Assoc"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000084754"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2009.04.006"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1645\/GE-883R.1"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/1801436a0"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1645\/GE-401R"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.04.001"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.1998.0426"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00411-4"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00507-7"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7375-7380.2005"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00258-5"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.1998.00161.x"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.8.4521-4527.2004"},{"key":"e_1_2_6_38_1","doi-asserted-by":"crossref","first-page":"834","DOI":"10.4049\/jimmunol.158.2.834","article-title":"Intranasal immunization with CTL epitope peptides from HIV\u20101 or ovalbumin and the mucosal adjuvant cholera toxin induces peptide\u2010specific CTLs and protection against tumor development in vivo","volume":"158","author":"Porgador A","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-8244(03)00202-5"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2006.11.002"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.05.013"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(02)02324-3"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01780-06"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182011001156"},{"key":"e_1_2_6_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.09.050"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.02.028"},{"key":"e_1_2_6_47_1","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1128\/CDLI.7.6.893-898.2000","article-title":"Immunization of BALB\/c mice with killed Neospora caninum tachyzoite antigen induces a type 2 immune response and exacerbates encephalitis and neurological disease","volume":"7","author":"Baszler TV","year":"2000","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_6_48_1","doi-asserted-by":"publisher","DOI":"10.2307\/3284489"},{"key":"e_1_2_6_49_1","doi-asserted-by":"publisher","DOI":"10.1053\/jcpa.2001.0539"},{"key":"e_1_2_6_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00088-6"},{"key":"e_1_2_6_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4922(02)02372-3"},{"key":"e_1_2_6_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.617-621.2005"},{"key":"e_1_2_6_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2011.09.008"},{"key":"e_1_2_6_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2011.01049.x"},{"key":"e_1_2_6_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2006.00427.x"},{"key":"e_1_2_6_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1037"},{"key":"e_1_2_6_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2008.01044.x"},{"key":"e_1_2_6_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2011.01065.x"},{"key":"e_1_2_6_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni904"},{"key":"e_1_2_6_60_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C600321200"},{"key":"e_1_2_6_61_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI43786"}],"container-title":["Parasite Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpim.12013","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pim.12013","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T10:15:30Z","timestamp":1744280130000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pim.12013"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,13]]},"references-count":60,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1111\/pim.12013"],"URL":"https:\/\/doi.org\/10.1111\/pim.12013","archive":["Portico"],"relation":{},"ISSN":["0141-9838","1365-3024"],"issn-type":[{"value":"0141-9838","type":"print"},{"value":"1365-3024","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12,13]]}}}