{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:06:56Z","timestamp":1791173216056,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"3-4","license":[{"start":{"date-parts":[[2013,2,5]],"date-time":"2013-02-05T00:00:00Z","timestamp":1360022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Technology R & D Programme of China","award":["No. 2008BAD96B11-3 & 2007BAD40B05"],"award-info":[{"award-number":["No. 2008BAD96B11-3 & 2007BAD40B05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Toxoplasmosis is a world\u2010wide zoonosis that causes significant public health and veterinary problems. The study of vaccines remains the most promising method for the future prevention and control of toxoplasmosis. Recombinant<jats:italic>Toxoplasma gondii<\/jats:italic>cyclophilin has been shown to have potent PPIase and<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201012\u2010inducing activities, thus promoting the stabilization of<jats:italic>T. gondii<\/jats:italic>'s life cycle and maintaining the survival of its host during evolution. In this study, the<jats:italic>T. gondii<\/jats:italic>cyclophilin gene was used to construct a<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>vaccine (<jats:styled-content style=\"fixed-case\">pVAX1<\/jats:styled-content>\u2010TgCyP). The immune response and protective efficacy of the vaccine against<jats:italic>T. gondii<\/jats:italic>infection in BALB\/c mice were evaluated. All<jats:styled-content style=\"fixed-case\">BALB\/c<\/jats:styled-content>mice that were vaccinated with<jats:styled-content style=\"fixed-case\">pVAX1<\/jats:styled-content>\u2010TgCyP developed a high response with TgCyP\u2010specific antibodies, and significant splenocyte proliferation (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00\u00b705) compared with<jats:styled-content style=\"fixed-case\">pVAX1<\/jats:styled-content>vector and PBS groups.<jats:styled-content style=\"fixed-case\">pVAX1<\/jats:styled-content>\u2010TgCyP also induced a significant Th1 type immune response, indicated by the higher production of<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20102 and<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00\u00b705). The survival rate of<jats:styled-content style=\"fixed-case\">BALB\/c<\/jats:styled-content>mice increased significantly after vaccination with<jats:styled-content style=\"fixed-case\">pVAX1<\/jats:styled-content>\u2010TgCyP (37\u00b75%) (<jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00\u00b705). These results indicate that TgCyP is a highly efficacious vaccine candidate that can generate protective immunity against<jats:italic>T. gondii<\/jats:italic>infection in<jats:styled-content style=\"fixed-case\">BALB\/c<\/jats:styled-content>mice.<\/jats:p>","DOI":"10.1111\/pim.12024","type":"journal-article","created":{"date-parts":[[2012,12,28]],"date-time":"2012-12-28T02:30:03Z","timestamp":1356661803000},"page":"140-146","source":"Crossref","is-referenced-by-count":20,"title":["The protective effect of a<scp>DNA<\/scp>vaccine encoding the<i>Toxoplasma gondii<\/i>cyclophilin gene in<scp>BALB\/c<\/scp>mice"],"prefix":"10.1111","volume":"35","author":[{"given":"P.","family":"Gong","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q.","family":"Yu","sequence":"additional","affiliation":[{"name":"Jilin Provincial Animal Disease Control Centre Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Ren","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine Jilin University Changchun China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1128\/iai.61.4.1169-1172.1993","article-title":"Toxoplasma gondii: a protozoan for the nineties","volume":"61","author":"Joiner KA","year":"1993","journal-title":"Infect Immun"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2002.00485.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.64.3.607-623.2000"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"287","DOI":"10.2460\/javma.1990.196.02.287","article-title":"Toxoplasmosis and other causes of abortions in sheep from north central 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